Department of Chemistry

James D. Burgess

Associate Professor of Chemistry

216.368.4490      jdb22@case.edu      Millis 225A

Interests: Analytical Chemistry, Biochemistry, Biophysical Chemistry, Materials, Medicinal Chemistry, Bio-Inorganic Chemistry, Electrochemistry

B.A. Longwood College, 1992
Ph.D. Virginia Commonwealth University, 1997
Postdoctoral, Iowa State University, 1998


Research focuses on the modification of electrode surfaces with biological molecules for both sensing and fundamental studies. Current areas of interest include electrochemical detection of cholesterol efflux from the cell plasma membrane (in vivo and in vitro) and microfluidic electrochemical sensor platforms.

Characterization of the cholesterol content of cell plasma membrane

The aim of this research is to develop electrochemical methodology that will investigate the cholesterol content of cell plasma membranes. The ability to evaluate the dynamics of intracellular cholesterol trafficking to and from the plasma membrane would allow characterization of pathways governing cholesterol homeostasis and, in particular, of the initial steps in atherogenesis. Our group has established a procedure for fabrication of an electrode-supported lipid bilayer membrane that allows immobilization of cholesterol oxidase, which could be employed to detect the cholesterol contained in the plasma membrane of a single cell. The steady-state electrode response observed appears to correlate with the cholesterol content of plasma membrane.


Handling single cells.



Selected Publications
  • Fang, D., Jiang, D., Lu, H., Chiel, H. J., Kelley, K. J., Burgess, J. D. "Observation of Cellular Cholesterol Efflux at Microcavity Electrodes" J. Am. Chem. Soc. 2009, 131, 12038-12039.
  • Jiang, D. C., Fang, D.J., Kelley, T. J., Burgess J. D. "Electrochemical Analysis of Cell Plasma Membrane Cholesterol at the Airway Surface of Mouse Trachea" Anal. Chem. 2008, 80: 1235–1239.
  • Jiang, D., Devadoss, A., Palencsár, S., Fang, D., White, N. M., Kelley, T. J., Smith, J. D., Burgess, J. D.. "Direct Electrochemical Evaluation of Plasma Membrane Cholesterol in live Mammalian Cells" J. Am. Chem. Soc. 2007, 129: 11352-11353.
  • Devadoss, A., Palencsár, S. M., Jiang, D., Honkonen, M. L., Burgess, J. D. "Microelectrode Detection of Cholesterol in Vesicle Lipid Bilayer Membranes" Anal. Chem. 2005, 77: 7393-7398.
  • Devadoss, A., Burgess, J. D. "Steady-State Detection of Cholesterol Contained in the Plasma Membrane of a Single Cell Using Lipid Bilayer-Modified Microelectrodes Incorporating Cholesterol Oxidase." J. Am. Chem. Soc. 2004, 126: 10214-10215.
  • Bokoch, M. P., Devadoss, A., Palencsár, M. S., Burgess, J. D. "Steady-State Oxidation of Cholesterol Catalyzed by Cholesterol Oxidase in Lipid Bilayer Membranes on Platinum Electrodes." Analytica Chimica Acta. 2004, 519: 47-55.
  • Devadoss, A., Burgess, J. D. "Detection of Cholesterol through Electron Transfer to Cholesterol Oxidase in Electrode-Supported Lipid Bilayer Membranes." Langmuir. 2002, 18: 9617-9621.
  • Wang, Y., Farrell, N., Burgess, J. D. "Direct Evidence for Preassociation Preceding Covalent Binding in the Reaction of cis-[Pt(NH3)2(H2O)2]2+ with Surface Immobilized Oligonucleotides." J. Am. Chem. Soc. 2001, 123: 5576-5577.


Collaborative Publications
  • Fang, D., West, R. H., Manson, M. E., Ruddy, J., Jiang, D., Previs, S. F., Sonawane, N. D., Burgess, J. D., Kelley, T. J. "Increased Plasma Membrane Cholesterol in Cystic Fibrosis Correlates with CFTR Genotype and Depends on de novo Cholesterol Synthesis" Respiratory Research 2010, 11:61.
  • Jiaxi Ding, Dechen Jiang, Michael Kurczy, Jennifer Nalepka, Brian Dudley, Erin I. Merkel, Forbes D. Porter, Andrew G Ewing, Nicholas Winograd, James Burgess, Kathleen Molyneaux. "Inhibition of HMGCoA Reductase Reveals An Unexpected Role for Cholesterol During PGC Migration in the Mouse" BMC Developmental Biology 2008, 8:120, 1-14.
  • White, N. M., Jiang, D. Burgess, J. D., Bederman, I. R., Previs, S. F., Kelley, T. J. "Altered cholesterol homeostasis in cultured and in vivo models of cystic fibrosis." American Journal of Physiology-Lung Cellular and Molecular Physiology 2007, 292: L476-L486.
  • Qiu, X., Lou, Y., Samia, A. C. S., Devadoss, A., Burgess, J. D., Dayal, S., Burda, C. "PbTe Nanorods via Sonoelectrochemistry" Angewandte Chemie, 2005, 44, 5855.
  • Steckel, J. S., Persky, N. S., Martinez, C. R., Barnes, C. L., Fry, E. A., Kulkarni, J., Burgess, J. D., Pacheco, R. B., Stoll, S. L. "Monolayer and Multilayer Films of [Mn12O12(O2CMe)16]" Nano Letters 2004, 4(3): 399-402.
  • Burgess, J. D., Hawkridge, F. M. "Direct Electron Transfer Reactions of Proteins and Enzymes at Electrodes" In Electroanalytical Methods of Biological Materials. A. Bajter-Toth and J.Q. Chambers, Eds. 2002, Mercel Dekker, Inc.