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DEPARTMENT OF OCCUPATIONAL AND ENVIRONMENTAL SAFETY

 

TABLE 3. COMPARISON OF BIOLOGICAL SAFETY CABINETS.

Type Face velocity (lfpm) Airflow Pattern Radionuclides/
Toxic Chemicals
Biosafety Level (s) Product Protection
Class I*, open front 75 In at front; out rear and top through HEPA filter No 2,3 No
Class II: Type A 75 70% recirculated through HEPA; exhaust through HEPA No 2,3 Yes
Type B1 100 30% recirculated through HEPA; exhaust via HEPA and hardducted Yes (Low Levels/volatility) 2,3 Yes
Type B2 100 No recirculation; total exhaust via HEPA and hardducted Yes 2,3 Yes
Type B3 100 Same as IIA, but plena under negative pressure to room and exhaust air is ducted Yes 2,3 Yes
Class III NA Supply air inlets and exhaust through 2 HEPA filters Yes 3,4 Yes