POSITION OBJECTIVE: Career development position for PhD or MD/PhD in the areas of vascular biology, thrombosis, and inflammatory disease
ESSENTIAL FUNCTIONS: Have the ability to perform relatively independent basic research in the general content area listed above. Have knowledge in mouse molecular genetics, mouse assays in thrombosis, angiogenesis, inflammation, and vascular biology. Have the ability to perform murine surgeries. Knowledge in cell culture and molecular biology, immunochemistry, enzymology, and protein biochemistry.
This individual should be able to keep an electronic notebook and write scientific papers in English.
NONESSENTIAL FUNCTIONS: Mentoring Research Assistants and pre-doctoral students.
Department: Continuous contact with department research staff to collaborate on research goals. Frequent contact with department administration to resolve any ordering or billing issues that may arise.
University: Frequent contact with collaborators outside of the department to develop and carry out research. Moderate contact with EHS, and ARC staff.
External: Moderate contact with collaborators outside of the university to develop research projects. Occasional contact with outside vendors for purchasing research materials.
Students: Regular contact with students in the lab.
SUPERVISORY RESPONSIBILITY Train and oversee rotation students, provide essential technical advice at bench to graduate students and take part in discussions with them.
QUALIFICATIONS Experience: Bachelor's degree and a PhD or MD/PhD in a biologic science. Must have the previous experience of publishing his/her science as a first author in scientific journal that has an Impact Factor rating.
- Ability to work well with others and to work independently strictly following directions and protocols.
- Ability to collect and analyze data with attention to detail and do multiple tasks simultaneously.
- Effective oral and written communication skills. Must be able to effectively communicate with the PI/supervisor as well as other lab members.
- Good interpersonal skills; ability to work and communicate with various individuals within and external to the University.
- Good organization skills; ability to multi-task, prioritize and meet deadlines. Must demonstrate attention to detail and accuracy and follow-through on assigned tasks.
- High degree of independence.
- Self-motivated with excellent problem solving skills. Must demonstrate good analytical skills, sound judgment and good decision-making.
- Ability to work effectively independently and collaboratively within a team.
- Ability to work with sensitive information and maintain confidentiality.
- Ability to rely on and follow instructions and pre-established guidelines to perform the functions of the job.
- Ability to operate laboratory equipment and learn to operate new instruments
- Familiarity with policies and procedures of lab and chemical safety
- Knowledge in Microsoft Office, Excel, PRISM and database applications, and with internet navigation.
- Willingness to learn new techniques, procedures, processes, and software programs as needed.
- Ability to organize, maintain and prepare accurate records The following qualities are also greatly valued: highly motivated, responsible, dependable and a self-starter; excellent oral and written communication skills and interpersonal skills; good organization skills; ability to multi-task, prioritize and meet deadlines; attention to detail and accuracy; ability to work effectively independently and collaboratively with others on and off campus.
- Extensive experience in Molecular Genetics, Molecular Biology, Cell Biology & Vascular Biology.
- Mouse breeding and genotyping to produce specific combinations of genes, double or triple knockout mice, inducible specific knockout, confirmation of germ line transmission in chimeric mice
- Surgical skills: mouse blood drawing, skin wounding, bone marrow isolation and tail vein injection (optional)
- Background for design and testing of peptides
- DNA and RNA isolation from blood, plasma, frozen tissue, cell culture and other samples using various techniques
- RNA expression analysis using real-time RT-PCR, SYBR Green PCR, and multiplex RT-PCR
- Molecular cloning amplification, Western blotting, Immunoprecipitation and Co-immunoprecipitation, culture of cancer cell lines, cell signaling experimental design, cell proliferation and apoptosis assays
- Isolation of leukocytes, platelets, and endothelial cells from murine blood
- Establishment of primary cell lines
- Leukocyte function assays: adhesion, chemotaxis, reactive oxygen species generation (optional but highly desirable)
- Site-directed mutagenesis, use of siRNA and CRISPR/Cas approaches to influence gene expression
- Knowledge of coagulation assays (optional)
- Image acquisition skills: regular microscope and confocal microscope operation.
- Computer software skills: Prism, Excel, Image J, Photoshop
Mostly, will work at bench performing experiments that requires long time standing and sitting. Will perform some experiments in the animal room, standing in the laminar flow hood. Involves walking between different buildings in the University. Will involve travel to meetings or conferences with funding. Must be willing to adjust working hours according to the nature of ongoing experiments and many times work extended hours to finish the experiments. Working on weekends and holidays are required depending on the ongoing experiments and need to accelerate data generation and data collections.
Interested candidates should email resumes or CVs to firstname.lastname@example.org.
In employment, as in education, Case Western Reserve University is committed to Equal Opportunity and Diversity. Women, veterans, members of underrepresented minority groups, and individuals with disabilities are encouraged to apply.
Case Western Reserve University provides reasonable accommodations to applicants with disabilities. Applicants requiring a reasonable accommodation for any part of the application and hiring process should contact the Office of Inclusion, Diversity and Equal Opportunity at 216-368-8877 to request a reasonable accommodation. Determinations as to granting reasonable accommodations for any applicant will be made on a case-by-case basis.