Working under general supervision, plan and carry out research projects in accordance with Divisional and PI needs. The Post-octoral fellow shall be responsible for rearing of and experimentation with mice under SPF conditions. They shall be responsible for isolating and culturing stem cells from different sources- mice and human tissue and ensuring the integrity of the experiments. They will also provide assistance to support the Division’s funding and manuscript preparation.
Perform research work in medical and related technical areas involves use of lab skills:
1. Plan and carry out the experiments related to isolating and culturing stem cells from different sources- mouse and human tissue. Perform mice intervention studies using intraperitoneal, tail vein and intra-arterial injection of stem cells. Utilize planning skills to ensure food and other necessary supplies are never in short demand to prevent exposing the colony to risky managerial conditions.
2. Conduct biological research including, but not limited to, tail bleeding, euthanasia, tissue collection and post-collection processing. RNA/DNA/Protein extraction required. Assess murine health and disease using imaging techniques like Magnetic resonance imaging and Bioluminescence.
3. Perform flow cytometry experiments.
4. Perform PCR, qPCR and Western Blot Analysis.
5. Assist Principal Investigator in testing hypotheses and validating lab-based experimental procedures. Handling of human samples may be required.
6. Collect experimental data using excellent record keeping practices (paper and electronic records), maintain documentation of all experiments/histological scoring records, and analyze data. Communicate results appropriately.
7. Oversee inventory of common laboratory reagents and buffers. Order reagents and prepare buffers when necessary.
8. Attend and participate in laboratory meetings. Provide technical suggestions.
9. Train new staff; and direct the work of students.
10. Record results for a particular experiment or closely related series of experiments. Maintain records of experiments
11. Write IACUC protocols.
Perform other duties as assigned.
Department: Daily contact with supervisor/Principal Investigator. Daily contact with other members of the laboratory.
University: Frequent interactions with other laboratories and with the Animal Resource Center staff.
External: Limited contact with vendors to exchange information.
Students: Contact with student employees.
This position has no direct supervision of staff employees. Train new staff; and direct the work of students.
Education/Experience: PhD degree (molecular biology, immunology, regenerative medicine) in an approved biotechnology program and 0 to 2 years of experience required. Prefer stem cell and flow cytometry experience.
1. Has knowledge of commonly-used concepts, practices, and procedures within experimental research, stem cells and animal husbandry.
2. Ability to perform rearing/experimentation of animals and injections (tail vein, intra-peritoneal and subcutaneous injections) in mice.
3. Relies on instructions and pre-established guidelines to perform the functions of the job.
4. Ability to operate laboratory equipment.
5. Must demonstrate compliance with CWRU animal research and care (ARC) policies and procedures and compliance to regulations of the Animal Welfare Act, Public Health Service Policy, AAALAC guidelines and other applicable regulatory guidelines.
6. Must demonstrate compassion for animals within CWRU facilities and dedication to the Animal Resource Center’s mission. Must handle animals with care and respect at all times.
7. Previous experience working with animals required.
8. Demonstrated ability to perform molecular imaging techniques and small animal procedures is preferred.
9. Excellent oral and written communication skills and interpersonal skills; must demonstrate the ability to effectively and professionally communicate and work with various individuals from a broad spectrum of disciplines, technical and educational backgrounds within the department, School and University, and with individuals outside the University.
10. Strong organization skills; ability to multi-task, prioritize and meet deadlines. Must demonstrate attention to detail and accuracy, time management skills, and follow-through. Must be willing to have emergency availability on a very occasional basis.
11. Proficiency in Microsoft Office and database management. Basic statistical knowledge and skills preferred.
General laboratory and vivarium environment. May have exposure to hazards. Handing of BSL-2 specimens/animals/projects is part of current job duties. Training will be provided as needed.
Case's animal facilities are accredited by the Association for the Assessment and Accreditation of Laboratory Animal Care (AAALAC) and is managed according to the "Guide for the Care and Use of Laboratory Animals” appropriate Federal Animal Welfare Regulations, and the Public Health Service “Policy on the Humane Care and Use of Laboratory Animals." This position, and all animal research personnel, are subject to internal compliance to SOM Animal Resource Center Standard Operating Procedures and to compliance regulations of the Animal Welfare Act, Public Health Service Policy, AAALAC guidelines, the State of Ohio Veterinary Practice Act, Federal Drug Enforcement Administration regulatory guidelines, US Food and Drug Administration Center for Veterinary Medicine regulations and other applicable regulatory guidelines.
Interested candidates can email a copy of their CV, Cover Letter and References to Alicia at firstname.lastname@example.org.
Case Western Reserve University provides reasonable accommodations to applicants with disabilities. Applicants requiring a reasonable accommodation for any part of the application and hiring process should contact the Office of Inclusion, Diversity and Equal Opportunity at 216-368-8877 to request a reasonable accommodation. Determinations as to granting reasonable accommodations for any applicant will be made on a case-by-case basis.
In employment, as in education, Case Western Reserve University is committed to Equal Opportunity and Diversity. Women, veterans, members of underrepresented minority groups, and individuals with disabilities are encouraged to apply.