Postdoctoral fellow - Medicine, Division of Infectious Diseases and HIV Medicine

Date: July 2022

Title: Postdoctoral fellow                    

Department: Medicine, Division of Infectious Diseases and HIV Medicine

School: Case Western Reserve University School of Medicine

Location: Biomedical Research Building, 10th Floor

Supervisor Name and Title: Stephen Carpenter, MD, PhD, Assistant Professor of Medicine

 

I. STATEMENT OF RESEARCH AND SCIENTIFIC RESPONSIBILITIES

The qualified candidate is responsible for performing and supervising research projects in the field of immunity to Mycobacterium tuberculosis. Specifically, the research will focus on characterizing the function of an array of human T cell responses to tuberculosis using primary human cells from blood and bronchoalveolar lavage (BAL) samples obtained from cohorts of healthy individuals previously infected with Mycobacterium tuberculosis and HIV. This includes experimental planning, data analysis, oral and written presentation of the results to the Faculty Mentor, collaborators or external parties, writing manuscripts for publications and grant proposals for funding from the main funding entities (NIH, NIAID) as well as foundations and private donors. Candidates should be highly experienced in standard immunology research techniques and data analysis. Strongest consideration will be give to candidates with experience in bioinformatics anlysis of next-generation sequencing data (RNA / DNA sequencing and single-cell RNAseq). 

As a post-doc, (s)he will work with a high level of independence, present data at departmental seminars and scientific meetings, prepare manuscripts, and apply for grant proposals. Managerial experience of technicians and junior trainees will be looked upon favorably. The position provides an opportunity to make exciting discoveries and impact the fields of infectious disease and immunology, which includes performing experiments in a biosafety level 3 (BSL-3) lab, working with human cells, and sequencing data analysis.

 

II. TECHNICAL SKILLS

  • A high level of experience with standard immunology techniques is essential. Required techniques include: immunomagnetic cell isolation, flow cytometry, ELISA/ELISPOT, fluorescent microscopy, western blotting, PCR and qPCR
  • Experience working with with human samples required
  • Knowledge and experience with a multitude of advanced techniques such as: PCR, RNA-seq, CRISPR / lentivirus design, immunohistochemistry staining and enzymatic activity assays.
  • Experience with data analysis systems such as: Prism, Flowjo, microsoft excel, R and required packages (e.g. Seurat) is required
  • Experience independently writing manuscripts for submission to peer-reviewed journals is required
  • Experience with analysis of next-generation sequencing data (DNA/RNAseq, single-cell RNA sequencing, TCR sequencing) is preferred

 

III. SUPERVISORY RESPONSIBILITIES

  • Supervise laboratory technicians/assistants and student trainees in experimental design and data analysis of a variety of projects.

 

IV. EDUCATION REQUIREMENTS

  • Minimum qualifications include a Ph.D. in immunology, microbiology or equivilent.
  • Prior post-doctoral research experience is preferred. 
  • Experience with in vitro human or murine live T cell assays, flow cytometry, and in vivo assays in mouse or non-human primate models is essential.
  • The successful candidate must have strong analytical, oral and written communication skills.
  • The successful candidate must keep organized records of experiments and analyzed results.

 

Send inquiries containing a cover letter, CV with list of publications and contact information for three references to: stephen.carpenter@case.edu