The responsibilities of this position include: the conception, design, execution, chemical/radioactivity assays, and data analysis of experiments involving the perfusion of isolated renal tubules from mice and rabbits. Some experiments will require mouse survival surgery aimed at injecting viral constructs into the kidneys of mice, with the goal of harvesting tubules from these kidneys several days later. Familiarity with basic principles of molecular biology would be helpful in ordering viral constructs for injection into mouse kidneys. The subjects of study include tubules from wild-type rabbits, wild-type mice, mice with the genetic deletion/modification of various genes of interest (e.g., RPTP, AT1A, ErbB1), or mice injected with the aforementioned viral constructs. These experiments require (1) the manual dissection of tubules from specific areas of the kidney; (2) the fabrication of precision glass pipettes tor tubular perfusion; (3) the fabrication of physiological saline solutions, including out-of-equilibrium (OOE) CO2/HCO3− solutions; (4) the actual perfusion of tubules in a microscope-based system; (5) the collection of fluid after it has passed through the tubule lumen; (6) the analysis of solutions using chemical and radioisotopic assays; (7) data analysis; and (8) paper writing. Finally, it is expected that the successful candidate will be able to read the literature and assist in the design of experiments.
1. Plan and carry out perfusion of isolated renal tubules from mice and rabbits (50%)
2. Carry out molecular biology and order viral constructs for injection into mouse kidneys (10%).
3. Maintain accurate and thorough records of experimental and other research data. (15%).
1. Plan and conduct physiology experiments in collaboration with the principal investigator (5%).
2. Train new staff and oversee the work of graduate students (5%).
3. Assist in developing improved techniques or procedures (5%).
4. Collect and provide data analysis (5%).
5. Perform other duties as assigned (5%).
Department: Daily contact with the principal investigator, research associates and students within the research group in the course of conducting research activities. Regular contact with principal investigators or laboratory personnel in other research groups or core facilities.
University: Occasional contact with personnel in other departments when using laboratory facilities that are shared between laboratories to share expertise or information.
External: Occasional contact with sales representatives for product inquiries or service.
Students: Daily contact with students in the laboratory to direct the work and/or make adjustments to procedures.
This position has no direct supervisions of staff employees. May share in the training of new staff and direct the work of students.
Education/Experience: PhD in physiology, biochemistry, pharmacology or related discipline and 1 to 3 years of laboratory experience.
1. Outstanding fine motor skills and manual dexterity.
2. Ability to maintain research focus on a technically challenging project.
3. Strong organization skills and maintain a laboratory notebook, attention to scientific detail.
4. Ability to multi-task, prioritize and give attention to detail in order to complete tasks and meet deadlines.
5. Ability to effectively operate laboratory equipment.
6. Ability to problem-solve effectively and assist in the development and implementation of alternative solutions as needed. Must demonstrate good judgment and logical decision-making.
7. Ability to work effectively, both independently and collaboratively within a team.
8. Technical competence with software programs such as Microsoft Office (Word, Excel, and PowerPoint) and scientific graphics package (such as Origin) is strongly preferred. Ability to learn and apply software programs and applications as needed.
9. Ability to learn and apply new techniques, ideas, processes, policies, functions, etc. Must be highly motivated and a self-starter.
10. Ability to conform to shifting priorities, demands and timelines. Must be flexible in order to respond to project adjustments and alterations promptly and efficiently. Must be able to adapt to varying workloads and respond appropriately to stressful situations.
11. Excellent verbal and written communication skills; good interpersonal skills with the ability to interact with colleagues, supervisors and customers face to face.
12. Ability to meet consistent attendance.
The employee will be working in a laboratory environment with general chemicals, and will need to take Chemical Safety Training. Required personal protective equipment includes a standard lab coat, gloves and safety glasses when appropriate.
In employment, as in education, Case Western Reserve University is committed to Equal Opportunity and Diversity. Women, veterans, members of underrepresented minority groups, and individuals with disabilities are encouraged to apply.
Case Western Reserve University provides reasonable accommodations to applicants with disabilities. Applicants requiring a reasonable accommodation for any part of the application and hiring process should contact the Office of Inclusion, Diversity and Equal Opportunity at 216-368-8877 to request a reasonable accommodation. Determinations as to granting reasonable accommodations for any applicant will be made on a case-by-case basis