Editing of the CFTR locus: the impact on chromatin architecture and gene expression
Department of Genetics and Genome Sciences Case Western Reserve University
Cleveland, Ohio. USA
The goals of this collaborative research program are to repair mutations in the cystic fibrosis transmembrane conductance regulator (CFTR) gene, by gene editing.
Errors in CFTR are associated with the common inherited disorder cystic fibrosis. CFTR has a complex pattern of tissue specific and temporal regulation (reviewed in Swahn and Harris, Genes 2019, 10, 235; doi:10.3390/genes10030235). The project will involve 1) CRISPR/Cas9 protocols to manipulate cis-regulatory elements in the CFTR locus and 2) Analysis of the impact of therapeutic gene editing on CFTR locus architecture and gene expression.
Successful applicants for this CFF funded program will have a strong record of accomplishment as shown by publications as a graduate student and/or postdoc, be highly motivated and have a passion for functional genomics research.
Expertise in one or more of the following areas is required: familiarity with use of public genome browsers and large data repositories; state of the art genomics technologies including ChIP-seq, RNA-seq, ATAC-seq, 4C-seq and the use of relevant bioinformatic pipelines; transcription factor biochemistry, CRISPR/Cas9 genetic manipulations.
Familiarity with general techniques of molecular biology and mammalian cell biology is essential. We are part of a vibrant and interactive research community at Case Western Reserve University and occupy excellent, modern laboratory space.
Please send an electronic letter of application, quoting the reference CWRU/AH1/2019, Curriculum Vitae and contact information of 3 referees to: firstname.lastname@example.org. Hiring is contingent upon eligibility to work in the United States.
DIVERSITY STATEMENT: In employment, as in education, Case Western Reserve University is committed to Equal Opportunity and Diversity. Women, veterans, members of underrepresented minority groups, and individuals with disabilities are encouraged to apply.
REASONABLE ACCOMMODATIONS: Case Western Reserve University provides reasonable accommodations to applicants with disabilities. Applicants requiring a reasonable accommodation for any part of the application and hiring process should contact the Office of Inclusion, Diversity and Equal Opportunity at 216-368-8877 to request a reasonable accommodation. Determinations as to granting reasonable accommodations for any applicant will be made on a case-by-case basis.