Date: October 2019
Title: Research Associate
Location: Wolstein Building
Supervisor Name and Title: Mark Jackson, Associate Professor Dept of Pathology
The qualified candidate is responsible for the supervision of research projects in the fields of oncology and onco-immunology (specifically preclinical models of immunotherapy of breast cancer and tumor-associated immune microenvironment in solid tumors) in all their aspects. This includes experimental planning, data analysis, oral and written presentation of the results to the Faculty Mentor, collaborators or external parties, writing and revision of manuscripts for publications on scientific journals and of grants proposals for funding from the main funding entities (NIH, NCI) as well as foundations and private donors. Mentoring and training of graduate students is required.
- Design and develop projects and experiments. Must have knowledge of the scientific significance of the project/experiments, according to the latest scientific discoveries in the field, identification of the finalities of the main project/s, including definition of priorities and resources (instruments, reagents, animals) required to complete the tasks.
- Conduct in vitro and in vivo experiments including eukaryotic and bacterial cell culture, nucleic acids and protein extraction, electrophoresis, proteomics, PCR (polymerase chain reaction) and real time qRT-PCR for DNA analysis, flow cytometry, animal microsurgery and pharmacological treatments as required by the project (injections – sub-cutaneous, intra-peritoneal, intra-venous-; tumor implant/explant), in vivo imaging, cell transfection, bright-field and fluorescence microscopy, confocal microscopy, histology and immunocytochemistry.
- Analyze data using specific programs and analysis procedures, including evaluation of reproducibility and reliability.
- Present project results to supervisors, collaborators, or external parties. Organization and presentation of data collected (as charts, figures, statistics), preliminary analysis of the results in correlation with the main finalities of the task/project, planning of future experiments/projects. Oral and poster presentations for meetings, including international conferences.
Write manuscripts/reviews and funding applications. Manuscripts for submission to scientific journals or reviews includes data selection and critical organization of the final manuscript. Manuscripts for peer-reviewed scientific publications must contain an introduction explaining the initial hypothesis, preliminary discoveries about the main topic, results supporting the main hypothesis, explanation/comment of the scientific importance of data collected. Writing literature reviews on specific topics as a single author or collaborator: requires a retrospective analysis of all the scientific literature about the main topic and a critical description of the major discoveries accomplished at the time of writing. Funding applications include critical organization of data and results in charts and figures, writing/revision of the final application or specific sections, critical and updated knowledge of the most recent discoveries in the field of interest.
- Assist collaborators or staff members in experiments planning or execution, decision-making, mentoring, development/optimization of instrument settings and work strategies.
- Instruct undergraduate students or employees of Case Western Reserve University, including training on specific techniques/instruments, mentoring during thesis or manuscript writing as needed.
- May supervise undergrads, technicians and direct the work of grad students.
- Organize shipment of samples from or to the laboratory.
- Assist technical staff during equipment maintenance and installation.
- Interact with the administrative office or other departments for issues regarding safety, budget, purchase orders and department organization.
- Perform other duties as assigned.
Dr. Mark W Jackson – FACULTY MENTOR – frequency 66% or more (frequent interaction) – updates about data collection and results, planning of future experiments and publications
Dr. Susan Pereira-Ribeiro – Instructor, Case Western Reserve University – COLLABORATOR -frequency 30% or more (regular interaction) – updates on current projects, troubleshooting, data/protocols sharing.
Dr. George R Stark – Full Professor, Lerner Research Institute at the Cleveland Clinic Foundation – COLLABORATOR – frequency 10% or more (regular interaction) – updates on current projects, troubleshooting, data/protocols sharing.
Department: Admin. Staff – Occasional
University: Environment health and safety, Animal Resource Center – Occasional
Students: Graduate and Undergraduate - Occasional
Experience: Expertise in the field of molecular medicine/oncology and immunology; ability to work with experimental animal models. Exceptional written and oral communication skills.
Education: PhD in Molecular Medicine/Molecular Oncology or equivalent degree.
- Knowledge and understanding of commonly used concepts, practices, and procedures within the field of molecular medicine and oncology.
- Knowledge and understanding of biology, biotechnology, in vitro and in vivo procedures
- Ability to maintain familiarity and demonstrate knowledge of scientific projects priorities, leadership and programs
- Ability to perform: cell cultures, main molecular biology and proteomics techniques, microscopy and imaging, FACS (fluorescence activated cell sorting), cell transduction and transfection, histology and immunohistochemistry, use of experimental animal models.
- Excellent oral and written communication skills and interpersonal skills; must demonstrate the ability to effectively and professionally communicate and work with various individuals from a broad spectrum of disciplines, technical and educational backgrounds within the department, School and University, and with individuals outside the University.
- Ability to operate laboratory equipment (cell culture hoods, spectrophotometer, flow cytometer, thermocycler, microscopes (bright-field, fluorescence, confocal), electrophoresis chambers, in vivo imaging systems, film developer and other instruments for protein imaging)
- Proficiency in Microsoft Office and specific programs: Image J, Metamorph, (image acquisition and analysis), Prism-GraphPad (statistical analysis), FacsDiva, FlowJo (FACS data acquisition and analysis), Living Image Software (in vivo imaging).
- Good/Strong organization skills; ability to multi-task, prioritize and meet deadlines. Must demonstrate attention to detail and accuracy, time management skills, and follow-through.
- Ability to work effectively independently and collaboratively within a team. (Must be highly motivated, responsible, dependable and a self-starter).
- Ability to work with sensitive information and maintain confidentiality.
- Effective problem-solving skills; must demonstrate sound judgment and good decision-making.
- Must demonstrate flexibility and ability to work under pressure; must be able and willing to conform to shifting priorities, demands and timeline.
- Willingness and ability to rely on and follow instructions and pre-established guidelines to perform the functions of the job.
- Must demonstrate willingness to learn new techniques, procedures, processes, and computer programs as needed.
Indoors. Biohazards and potentially toxic substances might be present in the environment (must follow safety instructions and training). Occasional travel and overtime work may be required.
In employment, as in education, Case Western Reserve University is committed to Equal Opportunity and Diversity. Women, veterans, members of underrepresented minority groups, and individuals with disabilities are encouraged to apply.
HOW TO APPLY
Qualified applicants should send their CV to firstname.lastname@example.org