Date: August 31, 2021
Title: Research Associate
Department: Medicine/Cardiovascular Medicine
Location: Wolstein Research Building
Supervisor Name and Title: Aaron Proweller, M.D., Ph.D.; Associate Professor
The Proweller laboratory broadly aims to identify novel mechanisms through which signaling in vascular smooth muscle (SM) influences angiogenesis and arterial vasoreactivity. Projects currently address the role of Notch signaling contribution in the adult mammalian vascular system. The work focuses more specifically on understanding how Notch in SM regulates arterial contractility and blood pressure with potential therapeutic implications to vascular hypertensive disease. Additional projects in the lab include the role of SM-derived VEGF in hypoxia induced angiogenesis.
Under the guidance of the PI, the Research Associate will be expected to work independently, read and critically interpret literature, design and plan projects, oversee collaborations, supervise students, manage animal models, and to carry out research activities through completion for the assigned project. The Research Associate will utilize mouse gene targeting in gain- and loss-of-function experiments and employ a wide array of cellular, molecular, vascular imaging and invasive hemodynamic monitoring techniques necessary for animal phenotyping. Specific methodologies include ex vivo arterial myography, in vivo hemodynamic analyses; in vivo and ex vivo angiogenesis assays, mouse surgery and tissue procurement; DNA, RNA, and protein-related assays (including ELISA); tissue culture, histological analyses; and mouse husbandry.
The Research Associate will present data at departmental seminars and scientific meetings, prepare manuscripts, and contribute preliminary data for grant proposals. The Research Associate is encouraged, however not required, to identify funding opportunities for the lab, including applying for applicable American Heart Association awards.
- Design, plan, and execute experiments for research projects within the lab and in collaboration with other teams. Oversee progress of the research projects and train lab staff and students on new and existing experimental techniques. Provide regular progress reports to the PI and provide recommendations based on analyses. (50%)
- Analyze and prepare results in publication quality figures. (10%)
- Present data, progress reports, and current literature to laboratory meetings and other scientific meetings. (10%)
- Independently write manuscripts and aid the PI in grant proposal submissions and publications. (10%)
- Mentor and train lab staff and graduate students; oversee research work and ensure projects are being completed according to research plan. (5%)
- Work with the PI to develop research protocols and independently develop and adapt laboratory techniques. (5%)
- Must demonstrate compliance with CWRU animal research and care (ARC) policies and procedures and compliance to regulations of the Animal Welfare Act, Public Health Service Policy, AAALAC guidelines and other applicable regulatory guidelines.
- Must demonstrate compassion for animals within CWRU facilities and dedication to the Animal Resource Center’s mission. Must handle animals with care and respect at all times.
- Must be able and willing to maintain a high standard of personal cleanliness and utilize protective gear to protect the health the animals.
- Assist with general lab operations including supply ordering, inventory, lab organization and maintain certain equipment/facilities. Assist with management of lab waste and chemical/safety procedures and protocols. (5%)
- Perform other duties as assigned. (5%)
Department: Frequent contact with the Principal Investigator for mentorship, regular contact with lab members; Contact with administrative staff as needed to obtain administrative support for research activities.
University: Routine contact with Environmental Health and Safety (EHS), as well as Animal Research Center (ARC) in order to meet regulatory requirements
External: Continuous contact with potential collaborators to further our and their research objectives.
Students: Regular contact with graduate and undergraduate students as part of the teaching mission.
No direct supervisory responsibilities. Will provide mentorship to students in the lab.
Experience: Prior post-doctoral research experience is not required, though experience in contemporary techniques of molecular biology and mouse handling/experimentation is essential.
Education/Licensing: Ph.D. in biology, physiology, chemistry, or equivalent. A background in vascular physiology is highly preferred.
REQUIRED KNOWLEDGE, SKILLS and ABILITIES
- Effective oral and written communication skills. Must be able to effectively communicate with the PI/supervisor as well as other lab members.
- Good interpersonal skills; ability to work and communicate with various individuals within and external to the University.
- Good organizational skills; ability to multi-task, prioritize and meet deadlines. Must demonstrate attention to detail and accuracy and follow-through on assigned tasks.
- Technical expertise required in biology lab work, cell culture, and mouse work including injections, treatments and survival surgery.
- Ability to work effectively independently and collaboratively within a team.
- Ability to work with sensitive information and maintain confidentiality.
- Good problem-solving skills; must demonstrate good analytical skills, sound judgment and good decision-making. Must demonstrate flexibility and ability to work under pressure; must be able and willing to work in a fast-paced, changing environment, and conform to shifting priorities, demands and timeline
- Ability to rely on and follow instructions and pre-established guidelines to perform the functions of the job.
- Ability to operate laboratory equipment and learn to operate new instruments.
- Familiarity with policies and procedures of lab and chemical safety.
- Knowledge in Microsoft Office, Adobe Photoshop/Illustrator, NIH Image J, GraphPad Prism, database applications, and internet navigation.
- Willingness to learn new techniques, procedures, processes, and software programs as needed
The researcher will work at the bench performing experiments that require standing and sitting. Will perform necessary activites in the animal room and tissue culture room, standing/sitting at the laminar flow hoods. Will involve travel to meetings or conferences if secured travel funding. Must be willing to adjust working hours according to the nature of ongoing experiments.
Case's animal facilities are accredited by the Association for the Assessment and Accreditation of Laboratory Animal Care (AAALAC) and is managed according to the "Guide for the Care and Use of Laboratory Animals” appropriate Federal Animal Welfare Regulations, and the Public Health Service “Policy on the Humane Care and Use of Laboratory Animals." This position, and all animal research personnel, are subject to internal compliance to SOM Animal Resource Center Standard Operating Procedures and to compliance regulations of the Animal Welfare Act, Public Health Service Policy, AAALAC guidelines, the State of Ohio Veterinary Practice Act, Federal Drug Enforcement Administration regulatory guidelines, US Food and Drug Administration Center for Veterinary Medicine regulations and other applicable regulatory guidelines.
In employment, as in education, Case Western Reserve University is committed to Equal Opportunity and Diversity. Women, veterans, members of underrepresented minority groups, and individuals with disabilities are encouraged to apply.