Under supervision, the Research Associate will work in a translational lab studying central nervous system (CNS) malignancies. The incumbent will work with a variety of human and mouse tissue samples and established human and mouse cell lines to study the onset, progression, and treatment of CNS malignancies. Incumbent will independently perform in vivo and in vitro assays such as flow cytometry, qPCR, lentiviral work, train new lab members, plan experiments, analyze data, and contribute to scientific papers. Incumbent will keep detailed records of all aspects of experimental data. Incumbent will assist in data preparation and drafting of manuscripts.
1. Develop the design and execution of experiments relating to efficient targeting of cancer stem cells (60%).
2. Lead effort to write manuscripts describing new research efforts (20%).
3. Contribute to the development of new grant proposals (10%).
4. Present research findings at conferences (5%).
5. Training and supervision of various post-doctoral, doctoral and medical students who may rotate through the laboratory (5%).
1. Theoretical knowledge of normal and malignant neural stem and progenitor cell biology sufficient to aid in the design and interpretation of experiments on those topics is preferred.
2. Perform other duties as assigned.
This section is important in rating the position, so please give it thought and consideration when developing the position description. Indicate who the incumbent will have contact with (i.e., department staff, students, potential donors), the frequency (i.e., infrequent (up to 5%), occasional (6-15%), moderate (16-30%), regular (31-45%), frequent (46-65%), and continuous (66+%) and for what purpose (i.e., provide information, resolve complaints, solicit donations).
Department: Occasional contact with multiple departmental faculty, staff, and students in order to provide information on research efforts.
University: Occasional contact with multiple faculty, staff, and students across the University and affiliates (UHCMC, CCF) to provide information on research efforts as normal for conducting collaborative research.
External: infrequent contact with external industrial partners as part of conducting collaborative research.
Students: Continuous contact with undergraduate and graduate students at CWRU. Occasional contact with students at other Universities in order to further research efforts.
Direct supervisory responsibility includes the authority to hire, transfer, suspend, layoff, recall, promote, discharge, assign, reward or discipline subordinate employees or effectively recommend such actions. List the job titles of the direct report under supervisory responsibility (refer to organization chart).
Supervise postdoctoral scholars, medical, graduate and/or undergraduate students during rotations in the lab as required.
Experience: At least 2 years of relevant experience in cancer stem cell biology with focus on translational cancer research is required.
Education/Licensing: MD or PhD degree required; Background in normal brain development strongly preferred.
1) Theoretical knowledge in molecular biology and biochemistry as typically attained by a Bachelor's degree, or an equivalent combination of education and experience.
2) Experience with all aspects of tissue culture including freezing, thawing, counting cells and splitting cultures.
3) Demonstrated skill in the operation and maintenance of various lab equipment such as centrifuges, Nanodrop, IVIS imaging machine, flow cytometer, and more.
4) Experience in caring for and handling small lab animals and in administering medications and anesthesia to perform surgical procedures on animals. Experience in euthanizing small laboratory animals. Familiarity with the writing of Animal Subjects protocols and with the surgical procedures. Willingness and ability to work with rodents and/or other lab animals and/or infected samples.
5) Demonstrated experience working with human tissues; tissue trimming, histology analysis, and knowledge in necropsy.
6) Experience with standard repetitive techniques that may be required in research protocols related to a specific lab. Demonstrated ability to read, interpret and apply protocols in conducting research. Demonstrated ability in following step wise experimental protocols. Skill to troubleshoot adaptations of experimental protocols.
7) Demonstrated experience reading and using Material Safety Data Sheets. Demonstrated ability to follow established Safety and Biohazardous Material Guidelines.
8) Demonstrated experience working in and maintaining a sterile environment.
9) Experience in the design, construction, production, purification, and use of viral gene transfer vectors, including adenoviral, lentiviral and retroviral vectors.
10) Demonstrated skill in accurate record keeping and ability to organize, analyze, and calculate data. Knowledge in basic math for maintaining ledgers, income and expenditure reports.
11) Experience with the operation of a personal computer and software application, such as Access, MS Word and Excel. Knowledge to work with and learn new software for analysis.
12) Manual dexterity and legible handwriting. Ability to assist with general lab maintenance, including cleaning and restocking supplies and managing a regular schedule in the lab.
13) Strong communication skills (both verbal and written) needed to interact professionally and effectively in the work environment, as well as effectively and accurately document research procedures. Ability to read, comprehend and discuss research materials.
14) Enthusiasm for learning new techniques and a genuine interest in scientific research. Willingness and ability to learn new skills, research methods, and scientific principles. Ability to train students on same.
15) Good organizational and time management skills.
16) Knowledge sufficient to coordinate studies with other departments and the lab. Skill to plan and organize work assignments in an efficient and productive manner.
17) Demonstrated ability to assist in the writing of papers for publication, research proposals.
18) Scientific knowledge of DNA plasmids and primers.
Identify the working conditions and physical demands which relate to the essential functions of the position, such as working indoors/outdoors, working with exposures to hazards, etc. Include special considerations for the position, such as occasional travel or necessary overtime.
Translational research environment includes interaction with physicians, engineers, physicists, and other medical professionals. May encourage some travel for conferences and special meetings (~1-2 weeks / year). May also require occasional overtime as needed for some in vivo experiments.
Interested applicants should reply to:
Eli E. Bar, PhD
Assistant Professor, Department of Neurological Surgery
Secondary Appointments in the Departments of
Neuroscience & Molecular Biology and Microbiology
School of Medicine, Case Western Reserve University
and the Case Comprehensive Cancer Center
Case Comprehensive Cancer Center
Wolstein Research Building Room 2-126 (Office), 2-304 (Lab)
2103 Cornell Rd, Cleveland, OH 44106
Case Western Reserve University provides reasonable accommodations to applicants with disabilities. Applicants requiring a reasonable accommodation for any part of the application and hiring process should contact the Office of Inclusion, Diversity and Equal Opportunity at 216-368-8877 to request a reasonable accommodation. Determinations as to granting reasonable accommodations for any applicant will be made on a case-by-case basis.
In employment, as in education, Case Western Reserve University is committed to Equal Opportunity and Diversity. Women, veterans, members of underrepresented minority groups, and individuals with disabilities are encouraged to apply.