Senior Research Associate - Department of Nutrition

Title: Senior Research Associate

Department: Nutrition

School: Medicine

Location: BRB 939

Supervisor Name and Title: John Tilton, MD, Associate Professor

 

POSITION OBJECTIVE

We currently have a Senior Research Associate position available in the laboratory of Dr. John Tilton at Case Western Reserve University. The Tilton laboratory is a collegial and well-funded lab investigating cutting-edge technologies for delivery of CRISPR-Cas9 genome editing complexes in vivo.

The successful candidate should hold a Ph.D. in biological sciences with research experience in molecular or cell biology, immunology, virology, or biochemistry. Working mostly independently, the Senior Research Associate will perform experimental work including PCR, recombinant DNA technology and cloning, cell culture, production and analysis of virus-like particles, western blots, gel electrophoresis, flow cytometry, and preparation of samples for mass spectrometry. The Senior Research Associate will also perform experimental work to develop and test strategies to deliver genome editing machinery to tissues in vivo in small animals. Experience with these techniques or animal work is desired. The Senior Research Associate will work closely with the principal investigator, laboratory members, and other individuals in the Department and School of Medicine. Generous financial compensation is provided in accordance with experience and qualifications. 

 

ESSENTIAL FUNCTIONS

  1. Plan and carry out experimental work of a complex and non-routine nature in accordance with the research studies. Experiments will include polymerase chain reaction (PCR) and Gibson assembly cloning of DNA plasmids; mammalian cell culture work including isolation, passaging, and transfection of cell lines and primary cells; production and purification of HIV and virus-like particles; cell line and primary cell infection experiments; and analysis of cells using western blotting, mass spectrometry, flow cytometry, ELISA and other methodologies. (40%)
  2. Plan and conduct experiments aimed at developing strategies to deliver protein and RNAs – including CRISPR/Cas9-gRNA complexes – to tissues in mice. Techniques include tail vein injection, genotyping using PCR, use of isoflurane anesthesia machines, managing and maintaining records of the mouse colony including monitoring breeding, and analysis of delivery using small animal imaging techniques including bioluminescence, fluorescence, micro-CT, and/or single photon emission computed tomography (SPECT) imaging (30%).
  3. Assist the principal investigator with dissemination of the research findings including authoring manuscripts and preparing data (figures) for publication, presenting research updates in the form of poster or oral presentations and national and international meetings.  (10%)
  4. Maintain accurate data recording and note-taking for experiments conducted. Analyze data and present findings at meetings with the principal investigator. (10%)
  5. Contribute to the day-to-day function of the lab including training new staff on standard operating protocols, working with students, and assisting with laboratory maintenance and oversight and maintenance of scientific reagents. (10%)

 

NONESSENTIAL FUNCTIONS

  1. Perform other duties as assigned.

 

CONTACTS

Department: Weekly contact with supervisor to discuss research. Daily contact with laboratory members including research associates and postdoctoral fellows, research assistants, and graduate and undergraduate students. Contact with other members of the Department when required for the effective completion of research objectives.

University: Occasional contact with lab personnel, department assistants, and principal investigators from other departments and centers across the CWRU campus, including particularly in the Departments of Molecular Biology and Microbiology, Pathology, and Genetics and Genome Sciences.

External: Contact with outside vendors as needed.

Students: Daily contact with student workers to assist with the training and oversight of students.

 

SUPERVISORY RESPONSIBILITY

As the senior research associate becomes established in the laboratory, they may be assigned research assistants to help them with their research. In this case, the research associate will have supervisory responsibilities along with the laboratory supervisor (Dr. Tilton).

 

QUALIFICATIONS

Experience: At least 5 years of research experience in cell or molecular biology, biochemistry, immunology, microbiology, or related fields.

Education/Licensing: Ph.D. degree in Microbiology, Immunology, Genetics, Biochemistry or other field in the biological sciences.

 

REQUIRED SKILLS

  1. Knowledge and understanding of common concepts, practices, and procedures within the field of cell or molecular biology, immunology, and biochemistry.
  2. Must demonstrate compliance with ARC policies and procedures and compliance to regulations of the Animal Welfare Act, Public Health Service Policy, AAALAC guidelines and other applicable regulatory guidelines.
  3. Must demonstrate compassion for animals within the ARC facilities and dedication to ARC’s mission.  Must handle animals with care and respect at all times.
  4. Knowledge of good laboratory skills and practices is required, specifically, knowledge of laboratory practices for cell culture, transfection, production of viruses and virus-like particles, and previous experience with mouse work are highly desirable.
  5. Experience with careful and safe handling of blood-borne pathogens and primary human cells. 
  6. Professional and effective verbal and written communication skills. 
  7. Good interpersonal skills including the ability to effectively work with various individuals throughout the Department, School, University and individuals external to the University. Ability to work collaboratively as part of a team in order to achieve a goal or complete a task.
  8. Experience in supervising students and technicians and coordinate laboratory and research activities is desirable.
  9. Strong organization skills; must be detail oriented, possess the ability to multi-task, prioritize and meet deadlines, and demonstrate attention to accuracy and have the ability to keep accurate and complete records.
  10. Ability to problem-solve effectively, and demonstrate good analytical skills, good judgment and logical decision-making. 
  11. Ability to work independently with limited supervision.  Must demonstrate a strong work ethic, self -motivation, and a commitment to high quality.
  12. Ability to carry out complex research assignments of a non-routine nature, including devising new protocols and techniques for research projects involving a high degree of skill and training.
  13. Strong computer skills, including proficiency with Mac and/or Windows operating systems, Microsoft Office (Word, Excel, Access and PowerPoint), and with internet navigation.  Ability to learn and apply software programs and applications as needed.

 

WORKING CONDITIONS

General laboratory environment.  This position will have contact with or exposure to the following hazards: chemicals and viruses.  Laboratory and bloodborne safety training is required and will be provided.  Appropriate personal protective equipment is also required and will be provided, including gloves, lab coat and protective eyewear. 

 

Case Western Reserve University’s animal facilities are accredited by the Association for the Assessment and Accreditation of Laboratory Animal Care (AAALAC) and is managed according to the "Guide for the Care and Use of Laboratory Animals” appropriate Federal Animal Welfare Regulations, and the Public Health Service “Policy on the Humane Care and Use of Laboratory Animals."  This position, and all animal research personnel, are subject to internal compliance to ARC SOP’s and to compliance regulations of the Animal Welfare Act, Public Health Service Policy, AAALAC guidelines, the State of Ohio Veterinary Practice Act, Federal Drug Enforcement Administration regulatory guidelines, US Food and Drug Administration Center for Veterinary Medicine regulations and other applicable regulatory guidelines.

 

DIVERSITY STATEMENT

In employment, as in education, Case Western Reserve University is committed to Equal Opportunity and Diversity.  Women, veterans, members of underrepresented minority groups, and individuals with disabilities are encouraged to apply.

 

Please send cv and the name of three references to Dr. John (Chip) Tilton at jct63@case.edu