Senior Research Associate - Department of Physiology & Biophysics

Sr. Research Associate

Department of Physiology & Biophysics

School of Medicine

 

 

POSITION OBJECTIVE

 

The successful candidate will be involved in research activities including but not limited to the following:

  1. Studying the activity of L-type calcium channel (LTCC) in adult cardiomyocytes and in vascular smooth muscle cells.
  1. From pending R01HL154038 grant application

Objective 1: Identifying the key functional group(s) in natural cyclic depsipeptides and their synthetic analogs that determine the inhibitory potency and selectivity towards Gq/11 class G proteins and LTCC. We will a) express functional LTCC subunits in cultured HEK cells; b) isolate primary cardio and vascular myocytes from adult wild type and Rgs KO mice; and c) use whole-cell electrophysiological recording of LTCC current in primary cardiomyocytes and in HEK cells to establish inhibitor potency and efficacy.

  1. From pending R01HL150117 grant application

Objective 1: Establishing how AT1R transactivation of EGFR and TGFbR signaling in the renal vasculature and epithelium of Eln+/- mice are involved in hypertension and renal fibrosis resulting from ELN deficiency. These objectives will be achieved in part by a) induction of nephron- and vascular smooth muscle-specific AT1R knockdown; isolation of renal cortical and medullary nephrons; c) isolation and culture of distal nephron epithelial cells; c) examination of renal tubular function and activity of Na+ transporters by single-channel electrophysiological assessment of ENaC activity.

 

  1. Studying the role of G protein signaling in excitation-contraction coupling in freshly isolated adult cardiomyocytes.
  2. Maintaining mouse breeding colonies.
  3. Participating in other related projects involving electrophysiology and transgenic mouse models.
  4. Training students and other research assistants of electrophysiology techniques.

 

ESSENTIAL FUNCTIONS

 

  1. Plan experiments, analyze data, write first drafts of manuscripts (including figure preparation), play similar roles in grant applications. 10%
  2. Set up mouse breeding pairs, wean pups, genotype offspring, maintain colony logs. 30%
  3. Perform electrophysiology experiments, as necessary, to achieve study or project objectives. 60%

 

NONESSENTIAL FUNCTIONS

  1. List duties that are marginal or infrequent.
  2. Perform other duties as assigned.
  1. Ensure laboratory is always up to date on safety and regulatory issues.
  2. Participate in writing IACUC protocols and amendments.

 

CONTACTS  

Department:

  • Laboratory PI: daily. Continuous (70%). To discuss plans and progress.
  • Mouse Physiological Phenotyping Core personnel (i.e., Director, staff scientists): weekly. Frequent (46%). To discuss plans and progress for managed projects and mouse colonies.
  • Administrative staff (particularly Administrative Director and Executive Assistant): To discuss stuff associated with department level (occasional, 10%) or arrangement of meetings with PI in laboratory (moderate, 20%).
  • Renal Epithelium (RE) Club (PI and RE Club lab members): biweekly for 90 min, regular (40%). To discuss plans and progress for associated projects.
  • Mouse Club (PI and Mouse Club lab members): biweekly for 90 min, regular (40%). To discuss plans and progress for managing mouse colonies with associated projects.
  • Lab Meeting (PI and all lab members): weekly for 120 min, regular (45%). To discuss plans and progress for associated projects and lab stuff.

University:

  • Administrative staff (particularly Director, Office of Postdoctoral Affairs): infrequent (up to 5%). To discuss stuff associated with university level.
  • Members of the RGS and G protein inhibitor ligand projects (Dr. Osei-Owusu, Alethia Dixon, Shelby Dahlen): monthly. Moderate (25%). To discuss plans and progress for LTCC experiments.
  • Members of the ELN project (Dr. Osei-Owusu, Dr. Ipsita Mohanty): monthly. Moderate (25%). To discuss plans and progress for expanding or paring down mouse colonies based on anticipated needs and experiments.
  • Students: Graduate (Alethia Dixon and Shelby Dahlen), frequent (50%).

External:

  • Members of the Moeller and Blumer Groups at Washington University in St. Louis (Prof. Kevin Moeller, Professor Ken Blumer, Dr. Ruby Kreuger, Carol Makepeace; inside department, Prof. Corey Smith, Prof. Jeff Garvin, Prof Walter Boron, and members of the Physiology and Biophysics Electrophysiology Core): biweekly for 60 min, regular (35%). To discuss plans and progress for projects involving electrophysiology and renal physiology.

 

SUPERVISORY RESPONSIBILITY

 

Manage the progress of mouse colonies and electrophysiology-related experiments being conducted in the Osei-Owusu lab.

 

QUALIFICATIONS

 

Experience: 5 years postdoctoral training and at least 15 years of experience in the cellular physiology of renal epithelial cells, cardiomyocytes, and vascular smooth muscle cells.

Education/Licensing: MD and/or PhD (in a physiology-related field)

 

 

REQUIRED SKILLS

.

  • Patch clamping techniques, including whole-cell and inside-out patching skills.
  • Familiarity with whole-mouse techniques: Understanding survival surgery. Isolation of adult cardiomyocytes, epithelial cells, and vascular smooth muscle cells. Measurement of urine osmolarity, arterial blood pressure, mouse genotyping.
  • Physiological chemistry: Ability to design physiological solutions. Deep understanding of osmolarity and acid-base balance.
  • Cell and Molecular biology (including Agarose gel electrophoresis, PCR, DNA/RNA extract, Isolation of genomic DNA, Regular and Real Time-PCR, Cloning, Western blot, Immunoprecipitation)
  • Morphology technology: Immunohistochemistry, Immunocytochemistry, digital confocal imaging, Immunofluorescence, Hematoxylin-eosin (HE) staining, blood smear.
  • Experimental animal models and gene knockout mouse management: Managing colonies of genetically modified mice. Ability to generate double KOs.
  • Mouse blood collection: submandibular bleeding and cardiac puncture.
  • Language: Fluency in spoken every-day and scientific English. Ability to read and write scientific English.
  • Software: Expertise with Microsoft Office, particularly Word, Excel, and PowerPoint. Expertise with Origin, Prism, DSI Ponemah, LabChart.

 

WORKING CONDITIONS

 

Working indoors and may work with exposures to biological hazards, etc. and will participate national/international meetings and conferences once a year regularly.

 

Please forward your CV to poseiowusu@gmail.com and mxs86@case.edu

 

In employment, as in education, Case Western Reserve University is committed to Equal Opportunity and Diversity. Women, veterans, members of underrepresented minority groups, and individuals with disabilities are encouraged to apply

Case Western Reserve University provides reasonable accommodations to applicants with disabilities. Applicants requiring a reasonable accommodation for any part of the application and hiring process should contact the Office of Inclusion, Diversity and Equal Opportunity at (216) 368-8877 to request a reasonable accommodation. Determinations as to granting reasonable accommodations for any applicant will be made on a case –by-case basis.