Senior Research Associate - Medicine; Division of Infectious Diseases

Date: May 2021

Title: Senior Research Associate

Department: Medicine; Division of Infectious Diseases

School: Medicine

Location: BRB, 10

Supervisor Name and Title: Carlos Subauste, M.D., Professor

 

POSITION OBJECTIVE

The Senior Research Associate will conduct, coordinate and supervise studies to examine host-pathogen interactions and the regulation of inflammation. Duties will include designing, planning and executing in vitro studies looking at the role of various surface and intracellular molecules on vesicular trafficking and killing of intracellular pathogens. He/she will design and conduct in vivo experiments examining the effect of manipulation of intracellular signaling cascades on pro-inflammatory responses and assist in the identification of novel agents that will be tested in animal models of diabetes and inflammatory bowel disease.  The candidate will assist the principal investigator in the supervision of research assistants, students and post-doctoral fellows as well as ensure laboratory maintenance and operation. As a Senior Research Associate he/she will prepare manuscripts and contribute preliminary data, background and significance and experimental design for grant proposals.  He/she will present findings in scientific meetings.

 

ESSENTIAL FUNCTIONS

  1. Work closely with the principal investigator in the coordination of laboratory and research activities. Provide regular updates to the principal investigator on planning of experiments, recent results, developments that may require a change in the experimental plan, and monitoring of laboratory budget. Oversee all laboratory staff, students and post-doctoral fellows as well as train new staff and students. Serve as the lab manager and lead research projects in the absence of the PI. (40%) 
  2. Design, plan and conduct studies that include but are not limited to genetic and pharmacologic manipulation of cell signaling pathways, expression and localization of intracellular proteins that regulate vesicular trafficking, regulation of survival of intracellular pathogens, animal studies in models of toxoplasmosis and inflammatory disorders. This includes statistical analysis and generation of graphs that summarize the experimental results. This may result in co-authorship in research projects.  (50%)
  3. Devise new protocols and techniques for research projects involving a high degree of skill and training and evaluate adequacy of existing laboratory techniques. Perform quality control evaluation of new protocols and techniques.  (10%) 

 

NONESSENTIAL FUNCTIONS

  1. Oversee the maintenance of laboratory equipment, storage of critical supplies, and compliance with safety standards.
  2. Perform other duties as assigned.

 

CONTACTS

Department: Frequent contact with supervisor to discuss research and maintain workflow Continuous contact with other lab personnel to discuss research and maintain workflow

University: Occasional contact with Animal Resource Center and university employees to discuss research and maintain workflow

External: Occasional contact with vendors to exchange information

Students: Continuous contact with graduate and undergraduate students working in the laboratory

 

SUPERVISORY RESPONSIBILITY

The SRA will assist the principal investigator with direct supervision of graduate students, undergraduate students, technicians and post-doctoral fellows. Instruct new laboratory personnel and students on appropriate laboratory practices.

 

QUALIFICATIONS

Experience: At least 5 years of experience required in immunology, microbiology or cell biology are required.

Education/Licensing: Ph.D. or M.D. is required.

 

REQUIRED SKILLS

  1. Expertise in tissue culture, flow cytometry, ELISA, PCR, immunoblot, handling of mice and collection of mouse organs.
  2. Professional and effective verbal and written communication skills. Strong interpersonal skills; ability to work with, communicate and supervise other members of the laboratory.
  3. Strong organization skills; ability to multi-task, prioritize and meet deadlines.  Must demonstrate attention to detail and accuracy, time management, and follow-through as well as a good habit of maintaining a clean lab working environment
  4. Ability to organize, maintain and prepare accurate records.
  5. Strong analytical abilities; effective problem-solving skills; sound judgment and good decision-making.
  6. Effective leadership skills; ability to work effectively independently and collaboratively within a team; must be highly motivated, responsible, dependable and a self-starter.
  7. Ability to operate laboratory equipment.
  8. Ability to meet consistent attendance.
  9. Must demonstrate compliance with university animal research and care (ARC) policies and procedures and compliance to regulations of the Animal Welfare Act, Public Health Service Policy, AAALAC guidelines and other applicable regulatory guidelines.
  10. Must demonstrate compassion for animals within university facilities and dedication to the Animal Resource Center’s mission.  Must handle animals with care and respect at all times.
  11. Must be able and willing to learn new techniques, procedures, processes, and computer gear to protect the health of the animals.
  12. Must demonstrate flexibility and ability to work under pressure; must be able and willing to conform to shifting priorities, demands and timeline.
  13. Ability to work with sensitive information and maintain confidentiality
  14. Proficiency with Microsoft Office, Excel and GraphPad Prism is preferred. Experience using Adobe Photoshop, Illustrator, and FlowJo is a plus.
  15. Must have the ability to maintain meticulous, complete, and easily retrievable laboratory data.
  16. Must demonstrate willingness to learn new techniques, procedures, processes, and computer programs as needed.

 

WORKING CONDITIONS

General laboratory environment: The lab is an open floor plan with abundant bench space for animal handling and manipulations. Ample desk space with computers are also provided. The lab is equipped with two fume hoods for storage of hazardous and non-hazardous materials as well as several freezers. The cell culture room is equipped with 3 incubators, 2 culture hoods, and microscopes. Access to a multi-color Flow Cytometer, ELISA reader and real time PCR machine in a neighboring labs. The candidate should expect frequent interactions with lab members from that lab and must be willing to collaborate. Major physical demands include transferring animals between the lab and the animal holding facility, maintaining the animal colonies, as well as weekly changes of mouse/rat cages. The individual will be exposed and use to routine laboratory chemicals and solvents, and paraformaldehyde for tissue processing. This position requires handling of animal and tissues from animals and humans following Biosafety Level II practices.

Case Western Reserve University's animal facilities are accredited by the Association for the Assessment and Accreditation of Laboratory Animal Care (AAALAC) and is managed according to the "Guide for the Care and Use of Laboratory Animals” appropriate Federal Animal Welfare Regulations, and the Public Health Service “Policy on the Humane Care and Use of Laboratory Animals."  This position, and all animal research personnel, are subject to internal compliance to SOM Animal Resource Center Standard Operating Procedures and to compliance regulations of the Animal Welfare Act, Public Health Service Policy, AAALAC guidelines, the State of Ohio Veterinary Practice Act, Federal Drug Enforcement Administration regulatory guidelines, US Food and Drug Administration Center for Veterinary Medicine regulations and other applicable regulatory guidelines.

 

Applicants may send inquiries containing a Curriculum Vitae (CV) with a list of publications and contact information with references to the Principal Investigator, Carlos Subauste at CSS34@case.edu.