David R. Kaplan, MD, PhD

Professor Emeritus
Department of Pathology
School of Medicine
Immune Oncology Program
Case Comprehensive Cancer Center

Dr. David Kaplan graduated with an A.B. in Biology from the University of Chicago in 1974. He later went on to earn a Ph.D. in Immunology and a M.D. in Medicine. His residency was in Pathology at Washington University from 1980 until 1984. In 1984 he came to Case's Department of Pathology as an Assistant Professor. From 1984 to 2000, Dr. Kaplan directed the Immunology Laboratories at University Hospitals of Cleveland and since 2007 he has directed the Diagnostic Immunology Laboratory at University Hospitals Case Medical Center. In 2009 Dr. Kaplan became the Director of the Pathology Residency Program and in 2012 he was named to the Graduate Medical Education Council at University Hospitals. He is a Professor of Pathology at Case Western Reserve University.

Research Information

Research Interests

Flow cytometric analysis is a powerful technique for the single cell assessment of molecules expressed at low levels. The major deficiency of flow cytometry has been its relative insensitivity. Only molecules expressed in abundance have been readily observed. We have developed an enzymatic amplification procedure for flow cytometry that increases the fluorescence signal between 10 and 100 fold thereby producing a significant enhancement in the resolving power of the technique. Using this technique we have been able to detect the presence of molecules that could not be observed by the standard procedure. With enzymatic amplification staining, we have significantly enhanced the sensitivity of flow cytometric analysis for surface receptors and a variety of intracellular analytes such as cyclin molecules, phosphoantigens, transcription factors, mitochondrial molecules, apoptosis mediators, and RNA. Enzymatic amplification staining gives a resolving capability that is comparable to the sensitivity of western analysis.

Additionally, I have been interested in the reform of peer review involving the assessment of grant applications for funding. I have proposed several ideas in a series of published essays and experimental studies.


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Kaplan D, Smith D (2000). Enzymatic amplification staining for flow cytometric analysis of cell surface molecules. Cytometry 40, 81-85.

Kaplan, D, H Meyerson, K Lewandowska. High resolution immunophenotypic analysis of chronic lymphocytic leukemia cells by enzymatic amplification staining (EAS). Amer J. Clin. Path. 116:429-36, 2001.

Kaplan, D, D Smith, H Meyerson, N Pecora, K Lewandowska. CD5 expression by B lymphocytes and its regulation upon Epstein-Barr Virus transformation. Proc. Natl. Acad. Sci. USA. 98:13850-13853, 2001.

Kaplan, D, W Husel, K Lewandowska. CD34 expression on platelets. Platelets. 14:83-87, 2003.

Kaplan, D. Enzymatic amplification staining for single cell analysis: applied to in situ hybridization. J Immunol. Methods 283:1-7, 2003.

Kaplan, D, H Meyerson, W Husel, K Lewandowska, G MacLennan. D cyclins in lymphocytes. Cytometry 63A:1-9, 2005.

Meyerson, H, G MacLennan, W Husel, W Tse, H Lazarus, D Kaplan. D cyclins in CD5+ B cell lymphoproliferative disorders: cyclin D1 and cyclin D2 identify diagnostic groups and cyclin D1 correlates with ZAP-70 expression in chronic lymphocytic leukemia. Amer J Clin Path 125:1-10, 2006.

Kaplan, D, HJ Meyerson, X Li, C Drasny, F Liu, M Costaldi, P Barr, H Lazarus. Correlation between ZAP-70, phospho-ZAP-70, and phospho-Syk expression in leukemic cells from patients with CLL. Cytometry B 78:115, 2010.

Manson GV, Campagnaro E, Balog A, Kaplan D, Sommers SR, Fu P, Rajkumar SV, Lazarus HM. Secondary MGUS after autologous hematopoietic progenitor cell transplantation in plasma cell myeloma: a matter of undetermined significance. Biol Blood Marrow Transplant 17:970-978, 2011.

Kaplan D, Kaye N, Liu F, Fu P, Margevicius S, Meyerson HJ, Lazarus HM. The functional duality of HoxB4 in hematopoietic reconstituting cells. Cytometry A In press, 2012.

Kaplan, D. Reform of Peer Review at NIH The Scientist 19(17):10, 2005.

Kaplan, D. Point: Statistical analysis in NIH peer review--identifying innovation. FASEB J. 2007 Feb;21(2):305-8.

Kaplan, D, N Lacetera, C Kaplan. Sample size and precision in NIH peer review. PLoS ONE I3:e2761, 2008.

Kaplan, D. Bias at NIH. Scientific American February, 2012.

Lacetera, N, Kaplan C, Kaplan D. Measuring creativity: a YouTube study. IEEE Proceedings. In press, 2012.

Kaplan, D. Social Choice at NIH: The Principle of Complementarity. FASEB J. 25:3763-3764, 2011.