Indications: Measured enzymatically in blood or CSF as an index of impaired pyruvate metabolism due to defects of glucose oxidation (fed state) or gluconeogenesis (fasted). The ratio of lactate to pyruvate reflects the NAD/NADH ratio and is useful in distinguishing primary defects of pyruvate metabolism from defects of electron transport (or oxidation). Deproteinize immediately (in perchloric acid) to avoid artifacts of lactate formation in red cells or loss of pyruvate.
Method Description: Spectrophotometric enzymatic assay (lactate dehydrogenase)
Specimen |
Specimen Requirements |
Shipping Requirements |
Deproteinized Blood
or
CSF |
Collect venous or arterial blood without prolonged stress to the patient with use of tourniquet, if needed. As soon as the blood or CSF is obtained, measure exactly 1 ml (with tuberculin syringe or a pipette) and transfer immediately to 2 ml of 8% w/v perchloric acid*. Stopper tube and shake vigorously for at least 15 seconds. The sample is now stable for local transport to a laboratory where it can be centrifuged. Freeze clear supernatant in a fresh tube.
Minimum Amt Required: 1 ml |
Ship sample on dry ice. |
*Perchloric acid is prepared by mixing 7 ml of 70% perchloric acid and distilled water to make 100ml total volume. Refrigerate until ready to use.
Samples Accepted: Monday - Friday, 8:30 AM - 5:00 PM
Turn-Around-Time: 3 business days
CPT Code(s): 83605, 84210
Specimens can be sent via Federal Express, DHL, or UPS.
Special Instructions: Ship using Overnight Priority/Next Morning.
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