Pricing

Next-Gen Sequencing Services

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Prices listed include necessary reagents and processing fees for the run only. All samples run on the Illumina instruments must first undergo final library quality control (QC) performed by the Core. Library Prep, QC, and other services requested are not included the cost of the run and will be charged separately. Prices updated July 2017. 

NextSeq 550 v2.5 flowcells

Description: 400 million clusters (high output kits), 130 million clusters (mid output kits). 

All pricing listed is for all CWRU affiliates and non-profit affiliates. For "for-profit" pricing, contact Simone Edelheit.

Type of run # of Cycles

Mid Output (130M clusters)

High Output (400M clusters)
Single Read 75 bp $1,500 $1,900
       
Paired-end Run 75 bp $1,500 $3,400
  150 bp $2,400 $5,400

MiSeq – v2 & v3 flowcells

Description: One lane flowcell with maximum 15M read output (v2 reagents) or 25M read output (v3 reagents).

All pricing listed is for all CWRU affiliates and non-profit affiliates. For "for-profit," contact Simone Edelheit.

Run Type  MS v3 (25M clusters) MS v2 (15M clusters) MS v2 - Pico (4M clusters) MS v2 - Nano (1M clusters)
1 x 50 bp $1,100 $950 $700 $500
1 x 150 bp $1,100 $1,400 $700 $500
2 x 75 bp $1,100 $1,400 $700 $500
2 x 150 bp $1,995 $1,400 $700 $500
2 x 250 bp $1,995 n/a n/a n/a

DNA & RNA Library Prep Services

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Prices include necessary reagents, processing fees, and QC of starting material and final libraries. The cost of sequencing is not included and will be charged separately. Discounts on larger projects are only applied when samples are submitted on the same day. Prices updated July 2017.

All pricing listed is for all CWRU affiliates and non-profit affiliates. For profit, add 48.5%. Prices updated July 2017.

DNA Library Description Price / Library
TruSeq DNA PCR Free Library #1-4 $250
  Library #5-24 $125
TruSeq Custom Capture Exome <18 Libraries $250
  >18 Libraries prepared at same time $185
2-Step PCR (CRISPR) max. 192 samples per pool $10
Nextera XT DNA cost of reagents per library $70 / library for reagents
  cost per 48 samples for processing $100 / set of 48 processing
16S Microbiome Analysis V3/V4 region, cost per 96-well plate  $1450 / 96-well plate

 

RNA Library Description Price/Library
TruSeq Stranded Total RNA – RiboZero Gold Library #1-8 $250
  Library #9-96 $185
Low and Ultra-low input RNA Library Prep contact the Core TBD

Quality Control and Size Selection Services

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All pricing listed is for all CWRU affiliates and non-profit affiliates. For profit, add 48.5%. Prices updated July 2017.

Service Description Price
QUBIT concentration of total RNA, DNA, and/or prepared library $4.00/sample
DNA QC Agilent DNA HS Bioanalyzer Chip – incl. Qubit $16.00/sample
  eGel – incl. Qubit $6.00/sample
RNA QC Agilent Bioanalyzer Pico / Nano RNA Chip – incl. Qubit $16.00/sample
Final Library QC Agilent DNA HS Bioanalyzer – incl. Qubit (per library) $16.00/sample
  qPCR Analysis – incl. Qubit (per library and/or final library pool) $25.00/sample and/or pool
MiSeq Libary QC Run Illumina MiSeq v2 Library QC run $500.00/run
DNA Size Selection / Shearing Covaris S2 $20.00/tube
DNA Size Selection Pippin Prep - price per cartridge (1-5 samples) $75.00/cartridge
Pooling Mix up to 12 libraries with unique tags into one pool $25.00
  Mix up to 24 libraries with unique tags into one pool $50.00
  Mix up to 48 libraries with unique tags into one pool  $75.00
  Mix up to 96 libraries with unique tags into one pool $100.00
Bead Clean-up Final Library Bead Clean-up - incl. Qubit and Bioanalzyer $25.00/sample

Other Services

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For Illumina genotyping/gene expression projects prices include only processing fee. Reagents are extra and should be purchased directly through Illumina. Prices updated July 2017.

All pricing listed is for all CWRU affiliates and non-profit affiliates. For profit, add 48.5%.

Other Services Using Applied Biosystems 3730 DNA Analyzer

Service Details Price
 Sanger Sequencing per reaction $8.00
  per reaction – full 96 well plate $6.00
  EXO/SAP PCR clean-up $1.00
 Microsatellite/SNPs/SNaPshot per reaction $5.00
  per reaction – full 96 well plate $3.00

Other Services Using Illumina HiScan Analyzer

BeadArray Service Array Type (not all-inclusive) Price / Chip
Infinium Genotyping Arrays  HumanOmni5, HumanOmni2.5 Exome, HumanOmni Express, InfiniumHD iSelect… $250
 Infinium Methylation Arrays Infinium Methylations450K (incl. Zymo EZ DNA Methylation prep) $350