| Next-Gen Sequencing | Library Preparation | Quality Control |
| Illumina BeadArray | Cell Line Authentication | Sanger Sequencing |
Next-Gen Sequencing Services
NovaSeq X Plus 1.5B / 10B / 25B Flow cells Now Available!
The CWRU Genomics Core offers sequencing services on Illumina MiSeq, NextSeq 550, and NovaSeq X Plus instruments. Prices listed include necessary reagents and processing fees for the run only. All samples run on the Illumina instruments must first undergo final library quality control (QC) performed by the Core. Library Prep, QC, and other services requested are not included the cost of the run and will be charged separately. Pricing updated October 2023.
NovaSeq X - Plus
- 10 billion clusters across 8 lanes are available in two different kit options: 300 cycle and 100 cycle
- For smaller projects - select Shared Flow cell - 1.25B clusters per lane
- Any run type (up to 300 cycles) can be selected for the shared flow cell
- 1.5B and 25B flow cell options now available!
NextSeq 550
- Great for mid-range projects (130-400M reads)
MiSeq
- Best for 18S / ITS and amplicon sequencing
- Used as QC tool before sequencing larger throughput (Micro / Nano)
All pricing listed is for all CWRU affiliates and non-profit affiliates. For "for-profit" pricing, contact Simone Edelheit.
| Sequencing Strategy | # of Reads | Run Type | Pricing |
|---|---|---|---|
| NovaSeq X 1.5B - Shared F/C | 750M (1 lane, of 2) | ANY | $1,700 |
| NovaSeq X 1.5B - 100 cycle | 1.5B | PE 50 / 10X | $2,400 |
| NovaSeq X 1.5B - 300 cycle | 1.5B | PE 150 | $3,200 |
| NovaSeq X 10B - Shared F/C | 1.25B (1 lane, of 8) | ANY | $1,700 |
| NovaSeq X 10B - 100 cycle | 10B | PE 50 / 10X | $8,500 |
| NovaSeq X 10B - 300 cycle | 10B | PE 150 | $11,000 |
| NovaSeq X 25B - 300 cycle | 25B | PE 150 | $20,000 |
| NextSeq 550 High Output - 75 cycle | 400M | SR 75 | $1,900 |
| NextSeq 550 High Output - 150 cycle | 400M | PE 75 | $3,400 |
| NextSeq 550 High Output - 300 cycle | 400M | PE 150 | $5,400 |
| NextSeq 550 Mid Output - 150 cycle | 130M | SR 75 / PE 75 | $1,500 |
| NextSeq 550 Mid Output - 300 cycle | 130M | PE 150 | $2,300 |
| MiSeq v3 - 600 cycle | 25M | PE 300 | $2,400 |
| MiSeq v3 - 150 cycle | 25M | PE 75 | $1,500 |
| MiSeq v2 Micro - 300 cycle | 4M | PE 150 | $700 |
| MiSeq v2 Nano - 300 cycle | 1M | PE 150 | $500 |
Library Prep Services
The CWRU Genomics Core offers a wide range of library prep services for both RNA and DNA. Prices include necessary reagents, processing fees, and QC of starting material and final libraries. The cost of sequencing is not included and will be charged separately. Discounts on larger projects are only applied when samples are submitted on the same day. Pricing updated October 2023.
All pricing listed is for all CWRU affiliates and non-profit affiliates. For profit, add 60%.
| Library Type (DNA) | Description | Price / Library |
|---|---|---|
| Whole Genome Library - PCR Free | <24 samples | $125 |
| >24 samples | $100 | |
| Whole Genome Library | <24 samples | $100 |
| >24 samples | $75 | |
| Human Exome Library | <12 samples | $250 |
| >12 samples | $200 | |
| 16S / ITS / custom amplicon | Full plate (up to 96 reactions) | $1,750 |
| Half plate (up to 48 reactions) | $1,250 | |
| Amplicon - Index ONLY | Full plate (up to 96 reactions) | $1,250 |
| ChIP-seq / CUT&RUN | Submit targeted DNA | $175 |
| CRISPR-Cas 9 Validation | Pool up to 288 amplicons | $15 |
| Library Type (RNA) | Descriptions | Price / Library |
|---|---|---|
| Total RNA Library | Library #1-8 | $200 |
| Library #9-96 | $175 | |
| mRNA Library | Poly-A enrichment | $150 |
| RIP-seq | Submit enriched RNA | $200 |
| Small RNA-seq | Submit total or enriched RNA | $200 |
| 10X Single Cell Platform | Contact AFGC | mjc230@case.edu |
Quality Control Services
The CWRU Genomics Core accepts samples for DNA and RNA QC at various stages of processing. Samples are analyzed on the Agilent Fragment Analyzer using the appropriate kit or other specified instruments. Prices updated October 2023.
| Service | Description | Price |
|---|---|---|
| QUBIT | Concentration of total RNA, DNA, and/or prepared library | $5.00/sample |
| DNA QC | Agilent Fragment Analyzer – incl. Qubit | $18.00/sample |
| eGel – incl. Qubit | $7.00/sample | |
| RNA QC | Agilent Fragment Analyzer – incl. Qubit | $18.00/sample |
| Final Library QC | Agilent Fragment Analyzer – incl. Qubit | $18.00/sample |
| qPCR Analysis – incl. Qubit | $40.00/reaction | |
| MiSeq Library QC Run | Illumina MiSeq v2 Nano flowcell | $500.00/run |
| DNA Shearing | Covaris S2 | $20.00/tube |
| DNA Size Selection | Pippin Prep - price per cartridge (1-5 samples) | $75.00/cartridge |
| Pooling | Mix up to 12 libraries with unique tags into one pool | $25.00 |
| Mix up to 24 libraries with unique tags into one pool | $50.00 | |
| Mix up to 48 libraries with unique tags into one pool | $75.00 | |
| Mix up to 96 libraries with unique tags into one pool | $100.00 | |
| Bead Clean-up | Final Library Bead Clean-up - incl. Qubit and Bioanalzyer | $25.00/sample |
Illumina Genotyping/Methylation BeadArray Services
For Illumina genotyping/gene expression projects prices include only processing fee. Reagents are extra and should be purchased directly through Illumina. Prices updated October 2023.
| BeadArray Service | Description | Price / Chip |
|---|---|---|
| Infinium Genotyping Array | up to 12 samples per chip | $300 |
| up to 24 samples per chip | $400 | |
| Infinium MethylationEPIC Array | incl. Zymo EZ DNA Methylation Prep | $400 |
| FFPE processing | $200 |
Cell Line Authentication and Sanger Sequencing Services
Cell Line Authentication services are available using the Promega GenePrint 10 system. We also offer standard Sanger sequencing and genotyping/microsatellite services, all using the ABI 3730 DNA Analyzer.
| Service | Details | Price / Sample |
|---|---|---|
| Cell Line Authentication | Includes Final Report | $100.00 |
| Sanger sequencing | per reaction | $8.00 |
| per reaction – full 96 well plate | $5.00 | |
| EXO/SAP PCR clean-up | $1.00 | |
| Microsatellite/SNPs/SNaPshot | per reaction | $5.00 |
| per reaction – full 96 well plate | $3.00 |
All pricing listed is for all CWRU affiliates and non-profit affiliates. For profit, add 60%.