The Biomarker and Basic Science Laboratory is a 1,600 square foot shared wet lab facility that provides FPB School of Nursing students and investigators with the resources needed to successfully collect and store biological specimens, as well as perform common DNA, RNA, and protein analyses.
- A Thermo Scientific Sorvall Legend XTR refrigerated benchtop centrifuge for processing of blood products.
- A liquid nitrogen cryogenic storage system, as well as multiple Thermo Scientific Revco -80°C ultralow temperature freezers equipped with remote monitoring for storage of biological specimens.
- Multiple -20°C freezers and 4°C refrigerators equipped with desiccation units for storage of chemical and biological reagents.
- Small equipment for microcentrifugation, western blot, ELISA, nucleic acid isolation, conventional PCR, and gel electrophoresis.
- An Applied Biosystems Step-One thermal cycling system for quantitative gene expression analysis and genotyping assays.
- A MesoScale Discovery QuickPlex SQ120 system for multiplexed chemiluminescence immunoassays.
- A Fisher Scientific Multiskan microplate reader for colormetric immunoassays.
- A Thermo Scientific NanoDrop photospectrometer for nucleic acid quantification
- An Invitrogen E-Gel Imaging system, as well as a LI-COR cDigit scanner, for documentation of gels and blots.
The laboratory is directed by Dr. Grant O’Connell, a PhD-trained biomedical scientist with an extensive background in clinical biomarker research, and managed by Maria Stoll, a molecular biologist with decades of bench experience. These expertise can be leveraged by FPB School of Nursing students and investigators for pre/post award consultation regarding the design and implementation of projects involving collection and analysis of biological specimens.