Case Western Reserve University




Lloyd Culp

Professor Emeritus

Extracellular matrix adhesion of tumor cells

Office Phone: 216-368-3407
Office Fax: 216.368.3055


Our laboratory is studying the  molecular mechanisms of adhesion of  fibroblasts and neuronal cells, as well  as their malignant derivatives, to fibronectin extracellular matrices since these processes are critical in regulation of gene expression and in the metastatic spread of tumor cells. Studies are revealing a diversity of mechanisms by which tumor and particularly metastatic cells adhere and detach from matrices, as well as the roles of several different cell surface receptors in these processes. Molecular biological interest has focussed on regulation of fibronectin and receptor gene expressions for effecting adhesive events during tumor progression, as well as on the amplification/over-expression of the N-myc oncogene (for the neural cells) or the Ras oncogene (for the fibroblasts) as influencing metastasis and for their influence on matrix adhesion of tumor cell populations. Clonal dominance of highly selected tumor cell subsets and the earliest events in micrometastasis formation in target organs are also being evaluated with drug-resistance genes and three different histochemical marker genes, permitting ultrasensitive tracking of tumor cells during tumor development and metastasis.

Selected Publications

Holleran, J.L., Miller, C.J., Edgehouse, N.L., Pretlow, T.P., and Culp, L.A. Differential experimental micrometastasis to lung, liver, and bone with lacZ-tagged CWR22R prostate carcinoma cells. Clinical Exp. Met., 19: 17-24 (2002). [PubMed]

Culp, L.A., Lin, W.-c., Kogerman, P., Holleran, J.L., and Miller, C.J. Tumor-specific metastasis to lung using reporter-gene tagged tumor cells. In Lung Cancer: Methods and Protocols, Methods in Molecular Medicine Series, Vol. 1, Edited by Barbara Driscoll, Humana Press, Inc., Totowa, N.J., pp. 509-526 (2002).

Culp, L.A., Judware, R., Kogerman, P., Holleran, J.L. and Miller, C.J. matrix and matrix receptors: alterations during tumor progression--update 2000. In Encyclopedia of Cancer, Second Edition, Vol. I, Ed. J.R. Bertino and J. Wang, Academic Press, Inc., San Diego, CA, in press (2002).

Holleran, J.L., Miller, C.J., and Culp, L.A. Tracking micrometastasis to multiple organs with lacZ-tagged CWR22R prostate carcinoma cells. J. Histochem. & Cytochem., 48: 643-651 (2000). [PubMed]

Kogerman, P., Sy, M.-S., and Culp, L.A. Over-expressed human CD44s promotes lung colonization during micrometastasis of murine fibrosarcoma cells: facilitated retention in the lung vasculature. Proc. Natl. Accad. USA, 94: 13233-38 (1997). [PubMed]

Complete list of Publications