The Tissue Culture (TCH) Module will provide visual science investigators with facilities and technical assistance for the maintenance and propagation of ocular and other cell types. The hybridoma division is involved in the development of monoclonal antibodies to proteins and other antigens of relevance to ocular disease. Availability of primary and immortalized cell lines from normal and diseased humans is crucial for studies on the molecular basis of ocular pathologies. Services provided by the TCH core facility, such as, development, maintenance and provision of cultured cells is a cost-effective use of manpower and resources.
The broad functions of Tissue Culture Module are 1) isolation of primary cells from ocular tissue; 2) maintenance and passage of established cell lines, including storage; 3) large scale amplification of cell lines; 4) transient transfection of cells; 4) generation of stably transfected cell lines; 5) training in growth and maintenance of cells; and 6) strategic advice in planning experiments and determining their feasibility. Specialized cell types currently stored include human and bovine retinal capillary endothelial cells, human and bovine retinal capillary pericytes, a rat retinal Muller cell line, a human retinal pigmented epithelial cell line, human and rabbit corneal epithelial cells, a rat retinal ganglion cell line, human umbilical vein and aortic endothelial cells, and mouse and human lens epithelial cells and cell lines. Equipment used in the Tissue Culture Module includes: 6 single chamber water jacket CO2 incubators, Nikon TS 100 with Fluorescence and Phase, 3 biological safety cabinets classII, type A, 3 cryobiological storage vessels, Axmaxa Nucleofector II, and Hypoxia Chamber.
How to Utilize
1) For Hybridoma, contact Denice Major at 216-368-6385 or firstname.lastname@example.org
2) For Tissue Culture, contact Dawn Smith at 216-368-0790 or email@example.com
Cell maintenance and antibody production: $9.00/hour for members, $15.00/hour for non-members
Ram Nagaraj, Ph.D., Module Director
Departments of Ophthalmology and Visual Sciences
2085 Adelbert Road
Institute of Pathology, Room 311
Phone: (216) 368-2089