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Genomics Core

Pricing

Pricing

Next-Gen Sequencing Services

Prices listed include necessary reagents and processing fees. Library Prep and Quality Control (QC) fees are not included and will be charged separately.

HiSeq 2500 – High Output v4 flowcell

Description:  Eight lane flowcell with maximum 250M read output per lane. All High Output runs require a MiSeq Library QC run (2x26bp).

All pricing listed is for all CWRU affiliates and non-profit affiliates. For "for-profit," contact Simone Edelheit.

Type of run# of CyclesPrice / flowcell
Single Read run 50bp $7,000
  100bp $9,900
Paired-end Run 50bp $12,000
  75bp $14,200
  125bp

$19,000

Note: No single line pricing available.

 

HiSeq 2500 – Rapid Run v2 flowcell

Description: Two lane flowcell with maximum 150M read output per lane.

All pricing listed is for all CWRU affiliates and non-profit affiliates. For "for-profit," contact Simone Edelheit.

Type of run# of CyclesPrice / flowcellPrice/lane - split 2 lanes
Single Read run 50bp $1,750 $1,075
  100bp $2,500 $1,450
Paired-end Run 50bp $2,900 $1,650
  100bp $3,900 $2,150

 

MiSeq – v2 & v3 flowcells

Description: One lane flowcell with maximum 15M read output (v2 reagents) or 25M read output (v3 reagents).

All pricing listed is for all CWRU affiliates and non-profit affiliates. For "for-profit," contact Simone Edelheit.

MiSeq Kits 600 cycle kit v3 (2x300bp max) 150 cycle kit v3 (2x75bp max)300 cycle kit v2 (2x150bp max)50 cycle kit v2 (1x50bp max)
MiSeq v3 (25M reads) $1900 $1050 n/a n/a
MiSeq v2 (15M reads) n/a n/a $1200 $900
MiSeq Micro v2 (4M reads) n/a n/a $1050 n/a
MiSeq Nano v2 (1M reads) n/a n/a $800 n/a

DNA & RNA Library Prep Services

Prices include necessary reagents, processing fees, and QC of starting material and final libraries. The cost of sequencing is not included and will be charged separately. Discounts on larger projects are Onlyapplied when samples are submitted on the same day. 

All pricing listed is for all CWRU affiliates and non-profit affiliates. For profit, add 48.5%.

DNA Library DescriptionPrice / Library
TruSeq DNA PCR Free Library #1-4 $250
  Library #5-24 $125
TruSeq Custom Capture Exome <18 Libraries $250
  >18 Libraries prepared at same time $175
2-Step PCR (CRISPR) max. 192 samples per pool $10
Nextera XT DNA cost of reagents per library $70 / library for reagents
  cost per 48 samples for processing $100 / set of 48 processing

 

RNA Library DescriptionPrice/Library
TruSeq Stranded Total RNA – RiboZero Gold Library #1-8 $250
  Library #9-96 $185
TruSeq RNA Access  – for low input and FFPE RNAs Library #1-6 $250
  Library #7-24 $185

Covaris Shearing & Quality Control Services

ServiceDescriptionPrice
QUBIT total RNA, DNA, and/or prepared library $2.00
DNA QC Agilent DNA HS Bioanalyzer Chip – incl. Qubit $14.00
  eGel – incl. Qubit $4.00
RNA QC Agilent Bioanalyzer Pico / Nano RNA Chip – incl. Qubit $14.00
Final Library QC Agilent DNA HS Bioanalyzer – incl. Qubit (per library) $14.00
  qPCR Analysis – may require Qubit (per library and/or final library pool) $15.00
MiSeq Libary QC Run Illumina MiSeq v2 Library QC run – required for HOP runs (2x26bp) $900.00
DNA Shearing Covaris S2 $15.00

Other Services

For Illumina genotyping/gene expression projects prices include only processing fee. Reagents are extra and should be purchased directly through Illumina. 

All pricing listed is for all CWRU affiliates and non-profit affiliates. For profit, add 48.5%.

Other Services Using Applied Biosystems 3730 DNA Analyzer

ServiceDetailsPrice
 Sanger Sequencing per reaction $8.00
  per reaction – full 96 well plate $6.00
  EXO/SAP PCR clean-up $1.00
 Microsatellite/SNPs/SNaPshot per reaction $5.00
  per reaction – full 96 well plate $3.00

Other Services Using Illumina HiScan Analyzer

BeadArray ServiceArray Type (not all-inclusive)Price / Chip
Infinium Genotyping Arrays  HumanOmni5, HumanOmni2.5 Exome, HumanOmni Express, InfiniumHD iSelect… $250
 Infinium Methylation Arrays Infinium Methylations450K (incl. Zymo EZ DNA Methylation prep) $350
 Whole Genome Gene Expression  HumanHT-12 $150