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Biochemistry Department - Primary Faculty

Hung-Ying Kao, Ph.D.

Professor

Education

  • Ph.D.: University of Minnesota, 1996
  • Postdoc: The Salk Institute for Biological Studies, 2001

Research Interests

Our lab is interested in transcriptional regulation in human health and diseases. Currently, we have two ongoing projects:

  • 1. Investigating the function and regulation of promyelocytic leukemia protein (PML).
    PML is a tumor suppressor protein that controls many cellular processes including apoptosis, cell proliferation, senescence, and transcription. PML is subjected to several post-translational modifications such as phosphorylation, sumoylation, acetylation, ubiquitination, and isgylation. We are interested in the following questions:
  •   –How do translational modifications regulate PML function?
  •   –How do the post-translational modifications of PML cross-talk with each other?
  •   –How do PML target genes mediate its cellular function?
  • 2. Characterizing the biological function and biochemical activity of the chromatin regulator UHRF1 (Ubiquitin Like With PHD And Ring Finger Domains 1)
    The chromatin regulator UHRF1 possess unique DNA binding activity in that it binds preferentially with hemi-methylated DNA. Additionally, UHRF1 binds methylated histone H3 and possess E3 ubiquitin ligase activity. UHRF1 is highly expressed in many cancer types that include prostate, colon and breast cancers. Our lab is interested in:
    •   –Characterization of UHRF1 nucleic acid binding activity
    •   –The mechanism by which UHRF1 and its interacting proteins regulate the expression of its target genes
    •   –The role of UHRF1 and its target genes in tumorigenesis

Selected References

  • Huang W., Peng Y., Kiselar J., Zhao X., Albaqami A., Mendez D., Chen Y., Chakravarthy S., Gupta S., Ralston C., Kao H.Y., Chance M.R., Yang S.
    “Multidomain architecture of estrogen receptor reveals interfacial cross-talk between its DNA-binding and ligand-binding domains.”
    Nat Commun. 30;9(1):3520(2018). Read article in PubMedCentral
  • Zhang Y. Y., Tabataba H., Liu X. Y., Wang J. Y., Yan X. G., Farrelly M., Jiang C. C., Guo S. T., Liu T., Kao H. Y., Thorne R. F., Zhang X. D., and Jin L.
    “ACTN4 regulates the stability of RIPK1 in melanoma”
    Oncogene 37 (29): 4033-45 (2018).
  • Pan S. C., Li C. Y., Kuo C. Y., Kuo Y. Z., Fang W. Y., Huang Y. H., Hsieh T. C., Kao H. Y., Kuo Y., Kang Y. R., Tsai W. C., Tsai S. T., and Wu L. W.
    “The p53-S100A2 Positive Feedback Loop Negatively Regulates Epithelialization in Cutaneous Wound Healing”
    Sci Rep 8 (1): 5458 (2018). Read article in PubMedCentral
  • Hsu K. S. and Kao H. Y.
    “PML: Regulation and multifaceted function beyond tumor suppression”
    Cell Biosci 8: 5 (2018). Read article in PubMedCentral
  • Zhao X., Khurana S., Charkraborty S., Tian Y., Sedor J. R., Bruggman L. A., and Kao H. Y.
    “alpha Actinin 4 (ACTN4) Regulates Glucocorticoid Receptor-mediated Transactivation and Transrepression in Podocytes”
    J Biol Chem 292 (5): 1637-47 (2017). Read article in PubMedCentral
  • Hsu K. S., Zhao X., Cheng X., Guan D., Mahabeleshwar G. H., Liu Y., Borden E., Jain M. K., and Kao H. Y.
    “Dual regulation of Stat1 and Stat3 by the tumor suppressor protein PML contributes to interferon alpha-mediated inhibition of angiogenesis”
    J Biol Chem 292 (24): 10048-60 (2017). Read article in PubMedCentral
  • Hsu K. S., Guan B. J., Cheng X., Guan D., Lam M., Hatzoglou M., and Kao H. Y.
    “Translational control of PML contributes to TNFalpha-induced apoptosis of MCF7 breast cancer cells and decreased angiogenesis in HUVECs”
    Cell Death Differ 23 (3): 469-83 (2016). Read article in PubMedCentral
  • Zhao X., Hsu K. S., Lim J. H., Bruggeman L. A., and Kao H. Y.
    “alpha-Actinin 4 potentiates nuclear factor kappa-light-chain-enhancer of activated B-cell (NF-kappaB) activity in podocytes independent of its cytoplasmic actin binding function”
    J Biol Chem 290 (1): 338-49 (2015). Read article in PubMedCentral
  • Lim L. M., Zhao X., Chao M. C., Chang J. M., Chang W. C., Kao H. Y., Hwang D. Y., and Chen H. C.
    “Novel Vitamin D Receptor Mutations in Hereditary Vitamin D Resistant Rickets in Chinese”
    PLoS One 10 (9): e0138152 (2015). Read article in PubMedCentral
  • Kao H. Y.
    “The actinin family proteins: biological function and clinical implications”
    Cell Biosci 5: 48 (2015). Read article in PubMedCentral
  • Guan D. and Kao H. Y.
    “The function, regulation and therapeutic implications of the tumor suppressor protein, PML”
    Cell Biosci 5: 60 (2015). Read article in PubMedCentral
  • Guan D., Lim J. H., Peng L., Liu Y., Lam M., Seto E., and Kao H. Y.
    “Deacetylation of the tumor suppressor protein PML regulates hydrogen peroxide-induced cell death”
    Cell Death Dis 5: e1340 (2014). Read article in PubMedCentral
  • Guo S., Cheng X., Lim J. H., Liu Y., and Kao H. Y.
    “Control of antioxidative response by the tumor suppressor protein PML through regulating Nrf2 activity”
    Mol Biol Cell 25 (16): 2485-98 (2014). Read article in PubMedCentral
Hung-Ying Kao Faculty's publications at pubmed