Below you will find a list of the most commonly used technical protocols in the Basch Lab:
In Situ Hybridization in Frozen Sections
This is the current optimized protocol for DIG-labeled In Situ Hybridizations.
Fluorescent In Situ Hybridization
This is the current optimized protocol for fluorescent single and double In Situ Hybridization.
Electroporation of embryonic cochlea
Cochlea electroporation allow us to deliver DNA plasmids, siRNAs or mRNA to the developing organ of Corti. This protocol explains how we do it.