David T. Lodowski graduated in 1998 from Tulane University with a BS in cellular and molecular biology. This was followed by graduate studies at the University of Texas at Austin, where, under supervision of Dr. John Tesmer, he obtained his PhD (Structural Basis for the Regulation of GRK2 by Gβγ) in 2005. He followed this work with a postdoctoral fellowship in the laboratory of Dr. Krzysztof Palczewski at Case Western Reserve University examining the structural changes in bovine rhodopsin that accompany photoactivation and GPCR activation from 2005 to 2011. Currently he is an assistant professor in the Department of Nutrition Case Center for Proteomics and Bioinformatics at Case Western Reserve University where his laboratory focuses on the interactions of GPCRs with their downstream partners employing a combination of X-ray crystallography, electron microscopy, and structural mass spectrometry techniques.
We are interested in utilizing X-ray and electron microscopic technologies to study macromolecular complexes that underlie the activation of G protein-coupled receptors and G proteins. We also utilize Mass spectroscopy and other biophysical techniques to further understand the enzymology and kinetics of these processes.
Teaching Information
Courses Taught
Research Information
Research Projects
- Dynamics of GPCR signaling
- Biophysics of Biosensors for markers of hypoxia and fatigue
- Aquaporins as membrane gas channels
Recent Funding
Biosis AFRL -- associate project lead, 7/15/20-present
Awards and Honors
Publications
Recent Publications
- Du Y, Duc NM, Rasmussen SGF, Hilger D, Kubiak X, Wang L, Bohon J, Kim HR, Wegrecki M, Asuru A, Jeong KM, Lee J, Chance MR, Lodowski DT, Kobilka BK, Chung KY. Assembly of a GPCR-G Protein Complex. Cell. 2019 May 16;177(5):1232-1242.e11. doi: 10.1016/j.cell.2019.04.022. Epub 2019 May 9. PMID: 31080064; PMCID: PMC6763313.
- Jain R, Abel D, Rakitin M, Sullivan M, Lodowski DT, Chance MR, Farquhar ER. New high-throughput endstation to accelerate the experimental optimization pipeline for synchrotron X-ray footprinting. J Synchrotron Radiat. 2021 Sep 1;28(Pt 5):1321-1332. doi: 10.1107/S1600577521005026. Epub 2021 Jul 20. PMID: 34475281; PMCID: PMC8415340.
- Moss FJ, Mahinthichaichan P, Lodowski DT, Kowatz T, Tajkhorshid E, Engel A, Boron WF, Vahedi-Faridi A. Aquaporin-7: A Dynamic Aquaglyceroporin With Greater Water and Glycerol Permeability Than Its Bacterial Homolog GlpF. Front Physiol. 2020 Jun 30;11:728. doi: 10.3389/fphys.2020.00728. PMID: 32695023; PMCID: PMC7339978.
- Hughes TET, Lodowski DT, Huynh KW, Yazici A, Del Rosario J, Kapoor A, Basak S, Samanta A, Han X, Chakrapani S, Zhou ZH, Filizola M, Rohacs T, Han S, Moiseenkova-Bell VY. Structural basis of TRPV5 channel inhibition by econazole revealed by cryo-EM. Nat Struct Mol Biol. 2018 Jan;25(1):53-60. doi: 10.1038/s41594-017-0009-1. Epub 2018 Jan 1. PMID: 29323279; PMCID: PMC5951624.
- Hughes TET, Lodowski DT, Huynh KW, Yazici A, Del Rosario J, Kapoor A, Basak S, Samanta A, Han X, Chakrapani S, Zhou ZH, Filizola M, Rohacs T, Han S, Moiseenkova-Bell VY. Structural basis of TRPV5 channel inhibition by econazole revealed by cryo-EM. Nat Struct Mol Biol. 2018 Jan;25(1):53-60. doi: 10.1038/s41594-017-0009-1. Epub 2018 Jan 1. PMID: 29323279; PMCID: PMC5951624.
- Blankenship E, Vahedi-Faridi A, Lodowski DT. The High-Resolution Structure of Activated Opsin Reveals a Conserved Solvent Network in the Transmembrane Region Essential for Activation. Structure. 2015 Dec 1;23(12):2358-2364. doi: 10.1016/j.str.2015.09.015. Epub 2015 Oct 29. PMID: 26526852; PMCID: PMC4670590.
- Blankenship E, Vukoti K, Miyagi M, Lodowski DT. Conformational flexibility in the catalytic triad revealed by the high-resolution crystal structure of Streptomyces erythraeus trypsin in an unliganded state. Acta Crystallogr D Biol Crystallogr. 2014 Mar;70(Pt 3):833-40. doi: 10.1107/S1399004713033658. Epub 2014 Feb 22. PMID: 24598752; PMCID: PMC3949523.
- Ernst OP, Lodowski DT, Elstner M, Hegemann P, Brown LS, Kandori H. Microbial and animal rhodopsins: structures, functions, and molecular mechanisms. Chem Rev. 2014 Jan 8;114(1):126-63. doi: 10.1021/cr4003769. Epub 2013 Dec 23. PMID: 24364740; PMCID: PMC3979449.