David T. Lodowski, PhD

Assistant Professor
Department of Nutrition
School of Medicine
Assistant Professor
Center for Proteomics and Bioinformatics
School of Medicine
Assistant Professor
Department of Pharmacology
School of Medicine
Graduate Program Director
Systems Biology and Bioinformatics
School of Medicine
PhD Program Director
Department of Nutrition
School of Medicine

David T. Lodowski graduated in 1998 from Tulane University with a BS in cellular and molecular biology. This was followed by graduate studies at the University of Texas at Austin, where, under supervision of Dr. John Tesmer, he obtained his PhD (Structural Basis for the Regulation of GRK2 by Gβγ) in 2005. He followed this work with a postdoctoral fellowship in the laboratory of Dr. Krzysztof Palczewski at Case Western Reserve University examining the structural changes in bovine rhodopsin that accompany photoactivation and GPCR activation from 2005 to 2011. Currently he is an assistant professor in the Department of Nutrition Case Center for Proteomics and Bioinformatics at Case Western Reserve University where his laboratory focuses on the interactions of GPCRs with their downstream partners employing a combination of X-ray crystallography, electron microscopy, and structural mass spectrometry techniques.

Teaching Information

Courses Taught

SYBB 501. Systems Biology & Bioinformatics Journal Club
CBIO 455. Molecular Biology I

Research Information

Research Projects

  • Dynamics of GPCR signaling
  • Biophysics of Biosensors for markers of hypoxia and fatigue
  • Aquaporins as membrane gas channels

Recent Funding

Biosis AFRL -- associate project lead, 7/15/20-present

Awards and Honors

Mt. Sinai Scholar
2012

Publications

Recent Publications

  1. Huynh KW, Cohen MR, Jiang J, Samanta A, Lodowski DT, Zhou ZH, Moiseenkova-Bell VY.  Structure of the full-length TRPV2 channel by cryo-EM.Nat Commun. 2016 Mar 29;7:11130. doi: 10.1038/ncomms11130. PMID: 27021073
  2. Blankenship E, Vahedi-Faridi A, Lodowski DT. The High-Resolution Structure of Activated Opsin Reveals a Conserved Solvent Network in the Transmembrane Region Essential for Activation. Structure. 2015 Dec 1;23(12):2358-64. doi: 10.1016/j.str.2015.09.015. Epub 2015 Oct 29. PMID: 26526852
  3. Schmandt N, Velisetty P, Chalamalasetti SV, Stein RA, Bonner R, Talley L, Parker MD, Mchaourab HS, Yee VC, Lodowski DT, Chakrapani S. A chimeric prokaryotic pentameric ligand-gated channel reveals distinct pathways of activation. J Gen Physiol. 2015 Oct;146(4):323-40. doi: 10.1085/jgp.201511478. PMID: 26415570
  4. Lodowski DT, Miyagi M. Analysis of conformational changes in rhodopsin by histidine hydrogen-deuterium exchange. Methods Mol Biol. 2015;1271:123-32. doi: 10.1007/978-1-4939-2330-4_9. PMID: 25697521
  5. Blankenship E, Lodowski DT. Rhodopsin purification from dark-adapted bovine retina. Methods Mol Biol. 2015;1271:21-38. doi: 10.1007/978-1-4939-2330-4_2. PMID: 25697514
  6. Blankenship E, Vukoti K, Miyagi M, Lodowski DT. Conformational flexibility in the catalytic triad revealed by the high-resolution crystal structure of Streptomyces erythraeus trypsin in an unliganded state. Acta Crystallogr D Biol Crystallogr. 2014 Mar;70(Pt 3):833-40. doi: 10.1107/S1399004713033658. PMID: 24598752
  7. Ernst OP, Lodowski DT, Elstner M, Hegemann P, Brown LS, Kandori H. Microbial and animal rhodopsins: structures, functions, and molecular mechanisms. Chem Rev. 2014 Jan 8;114(1):126-63. doi: 10.1021/cr4003769. Epub 2013 Dec 23. Review. PMID: 24364740
  8. Golczak M, Kiser PD, Sears AE, Lodowski DT, Blaner WS, Palczewski K. Structural basis for the acyltransferase activity of lecithin:retinol acyltransferase-like proteins. J Biol Chem. 2012 Jul 6;287(28):23790-807. doi: 10.1074/jbc.M112.361550. Epub 2012 May 17. PMID: 22605381
  9. Salon JA, Lodowski DT, Palczewski K. The significance of G protein-coupled receptor crystallography for drug discovery. Pharmacol Rev. 2011 Dec;63(4):901-37. doi: 10.1124/pr.110.003350. PMID: 21969326
  10. Jastrzebska B, Ringler P, Lodowski DT, Moiseenkova-Bell V, Golczak M, Müller SA, Palczewski K, Engel A. Rhodopsin-transducin heteropentamer: three-dimensional structure and biochemical characterization. J Struct Biol. 2011 Dec;176(3):387-94. doi: 10.1016/j.jsb.2011.08.016. PMID: 21925606

Education

Doctor of Philosophy
Biochemistry
University of Texas at Austin
2005
Bachelor of Science
Molecular Biology
Tulane University
1999
Post Doctoral Training
Structural Biology of Vision
Palczewski Laboratory, Case Western Reserve University
2011

Residencies, Internships and Fellowships

Additional Information

Protein Structures Determined: