Masashi Tabuchi, PhD

Assistant Professor
Department of Neurosciences
School of Medicine

Research Information

Research Interests

How neurons generate information to drive behavior? The Tabuchi lab's goal is to understand how neural coding impacts molecular/cellular signaling, plasticity, and behavior. We apply multidisciplinary approaches in Drosophila to understand how non-canonical (temporal or analog) neural codes represent persistent internal drive. Our research scope includes hierarchical interactions of gene expression, biophysical properties such as ionic currents, temporal structure of membrane potential dynamics including spiking patterns, functional synaptic connectivity of neural circuits, and behavior, with fundamental questions of what is the determining factor of processing performance in a neural circuit, what kind of molecular and cellular events can be attributed to, and how exactly do these events have a certain power in modulating the neuronal, synaptic, and behavioral states of the animals. By answering these questions, we expect to gain key insights into the principles of neural codes underlying persistent internal drive of the brain and to provide a framework to understand how these factors ultimately contribute to our health.

Research Projects

1. Molecular and Neural Mechanisms of Sleep

We are interested in understanding the relationship between sleep quality and neural coding. I previously discovered that sleep quality is determined by clock-driven temporal coding in an arousal circuit.  I also delineated the molecular and biophysical mechanisms underlying the effect on downstream arousal circuits on temporal codes and identified a novel form of synaptic plasticity driven solely by the temporal pattern of spiking. Along this line of study, the lab studies how neural codes impact molecular/cellular signaling to regulate sleep quality by applying methods of in vivo intracellular and extracellular electrophysiology, in vivo functional imaging, optogenetics, and sophisticated behavioral analysis.

2. Molecular and Neural Mechanisms of Nutrition Specific Hunger

I previously identified the first neural circuit encoding protein-specific hunger (named DA-WED neurons) and found that DA-WED neurons are critical for protein hunger in Drosophila. Using a combination of molecular, genetic, computational, and electrophysiological approaches in Drosophila, we are interested in understanding how temporal structure of membrane potential is used as non-canonical (temporal or analog) neural codes in DA-WED neurons for the persistent internal drive of nutrition-specific hunger/satiety.

3. Molecular and Neural Mechanisms of Alzheimer's Disease

I previously investigated the relationship between sleep loss and neuronal excitability as well as Aβ accumulation by using Alzheimer's disease model of Drosophila, and found a causal link between sleep and Alzheimer's disease such that the effects of sleep on Alzheimer's disease pathology and lifespan are mediated by changes in neuronal excitability.  Along this line of study, the lab seeks to identify novel pathological mechanisms of Alzheimer's disease.

Visit the Tabuchi Lab

Awards and Honors

Outstanding Early Investigator Award
2019
Sleep Research Society
Daniel Nathans Young Investigator Award
2019
Johns Hopkins School of Medicine
Yoshida Prize for Young Researchers
2019
Japanese Society for Comparative Physiology and Biochemistry

Publications

View All Publications

Selected Publications

Xie X, Tabuchi M, Corver A, Duan G, Wu MN, Kolodkin AL. (2019). Semaphorin-2b Regulates Sleep Circuit Formation in the Drosophila Central Brain. Neuron 104(2):322-337.

Tabuchi M, Monaco JD, Duan G, Bell BJ, Liu S, Liu Q, Zhang K, Wu MN. (2018). Clock-Generated Temporal Codes Determine Synaptic Plasticity to Control Sleep. Cell 175(5): 1213-1227.

Liu Q*, Tabuchi M* (co-first author), Liu S, Kodama L, Horiuchi W, Daniels J, Chiu L, Baldoni D, Wu MN. (2017). Branch-Specific Plasticity of a Bifunctional Dopamine Circuit Encodes Protein Hunger. Science 356, 534-539.

Liu S, Liu Q, Tabuchi M, Wu MN. (2016). Sleep Drive Is Encoded by Neural Plastic Changes in a Dedicated Circuit. Cell 165(6): 1347-1360.

Tabuchi M, Lone SR, Liu S, Liu Q, Zhang J, Spira AP, Wu MN. (2015). Sleep Interacts with Aβ to Modulate Intrinsic Neuronal Excitability. Current Biology 25(6):702-712.
 

Education

Doctor of Philosophy
The University of Tokyo
2013
Postdoctoral Fellowship
Johns Hopkins University
2019