Development and biomedical application of chemical sensing schemes is the focus of my laboratory. Diagnostics in 1-20 microliter body fluids and other samples is performed using micro fabricated electrochemical and optical sensors and an electrochemical micro-pH-stat. We also developed an equivalent of rotating electrode for microliter samples, called the rotating sample system. For cellular research, a diffusional microburet has been developed to continuously deliver reagents and drugs into single live cells. Fluorescence microscopy is employed to quantitate intracellular accumulation. Efflux is measured with a carbon fiber based electrochemical microsensor that can detect Adriamycin, a cancer drug, extruded from cells at very low concentrations. Neurochemical studies as well as measurements at epithelial cell layers are also performed with different microscopic sensing schemes.
- Potdar AA, Sarkar J, Das NK, Gosh P, Gratzl M, Fox PL, Saidel GM: Computational modeling and analysis of iron release from macrophages. PLOS Computations Biology 12: 243-267 (2014)
- Ahuja P, Peshkova MA, Hemphill B, Gratzl M: Minimizing color interference from biomedical samples in optode-based measurements. Sensors and Actuators II Chemical (12): 212-216 (2014)
- Ahuja P, Nair S, Narayan S, Gratzl M: Functional imaging of chemically active surfaces with optical reporter microbeads. 10(9), PPLOS ONE (2015)