Miklos Gratzl, PhD

Development and biomedical application of chemical sensing schemes is the focus of my laboratory. Diagnostics in 1-20 microliter body fluids and other samples is performed using micro fabricated electrochemical and optical sensors and an electrochemical micro-pH-stat. We also developed an equivalent of rotating electrode for microliter samples, called the rotating sample system. For cellular research, a diffusional microburet has been developed to continuously deliver reagents and drugs into single live cells. Fluorescence microscopy is employed to quantitate intracellular accumulation. Efflux is measured with a carbon fiber based electrochemical microsensor that can detect Adriamycin, a cancer drug, extruded from cells at very low concentrations. Neurochemical studies as well as measurements at epithelial cell layers are also performed with different microscopic sensing schemes.