About the Case Center for Synchrotron Biosciences
What is a Synchrotron?
Synchrotrons are accelerator facilities that provide extremely high flux and high brightness electromagnetic radiation at energies ranging from the infrared, through the ultraviolet, to the X-ray regions for investigating the structure of matter. The storage ring at the NSLS is designed to maintain "bunches" of electrons in a circular orbit, the energies of 2.8 GeV on the X-ray ring and 750 million electron volts on the Vacuum Ultraviolet-Infrared ring. These electron bunches are maintained in the circular orbit by a series of bending magnets that are equally spaced around the 170 meter circumference of the ring. As the electrons traverse the magnetic fields, they are accelerated or "bent" to maintain the orbit, and in the process photon energy is given off over a wide frequency range. Thus, at each of the 40 bending magnets found around the ring (and at selected straight sections), beam ports are placed (up to three can co-exist side by side for each magnet) to allow the radiation to pass safely in a confined vacuum space to an experimental chamber. The photon energy spectrum given off depends on the characteristics of the bending magnet, and most beamlines on the X-ray ring provide X-ray radiation in the energy range from 4,000 to 20,000 electron volts (0.8-3Ã. wavelength). For biologists, this radiation provides opportunities to conduct experiments in X-ray Imaging, Crystallography and diffraction, small angle scattering, X-ray Spectroscopy, and X-ray Footprinting. The Vacuum Ultraviolet-Infrared ring is similar in overall design, but is smaller (51 meters circumference), operating at lower energies, and provides ultraviolet and infrared light.
The Synchrotron Biosciences Projects at CSB
The use of synchrotron technologies for research in the biomedical sciences at the NSLS has undergone rapid growth. The research activities at CSB are mainly dedicated to the fields of spectroscopy, hydroxyl radical footprinting and crystallography. X-ray absorption spectroscopy is used to provide high resolution structural and electronic state information about the active sites of metalloproteins. Crystallography is used to determine the three dimensional structures of macromolecules and nucleic acids at atomic resolution by structural genomics and structural biology investigators; hydroxyl radical footprinting coupled with mass spectrometry (for proteins) or gel analysis (for nucleic acids) is used to study the dynamics of large macromolecular complexes, protein-protein interactions and protein-nucleic-acid interactions.
A decade ago, the technologies and facilities suitable to study one protein at a time and one gene at a time were the state-of-the art. Now, novel high-throughput technologies have changed the situation where multiple genes and proteins can be studied simultaneously. CSB has been instrumental in the development and implementation of a number of novel technologies for high- throughput and quality research in the field of Structural & Molecular Biology. The goal of the CSB is to enhance the research efforts of in-house faculty and provide unique and powerful research tools to assist investigators across the US and around the world in pursuing their current grant programs as well as assisting them in the pursuit of new awards. Furthermore, the novel structural proteomics technologies developed at the CSB will be dedicated to core, collaborative and service projects for international community of biomedical scientists.
National Synchrotron Light Source II
Meeting the critical scientific challenges of our energy future will require advanced new capabilities that a new facility called NSLS-II will uniquely provide. NSLS-II will be a new state-of-the-art, medium-energy electron storage ring (3 billion electron-volts) designed to deliver world-leading intensity and brightness, and will produce x-rays more than 10,000 times brighter than the current NSLS.
With support from National Science Foundation and Case Western Reserve University, the Case Center for Synchrotron Biosciences team is embarking on a journey to develop and construct the next world-class instrument for synchrotron footprinting (FP), the XFP beamline. This beamline is expected to provide more than an order of magnitude of additional flux density for FP experiments, enabling study of more complex biological systems and allowing researchers to access timescales in the microseconds. Learn More