Using a combination of a Qubit Fluorometer, the Agilent TapeStation, and the Invitrogen E-gel Agarose Gel Electrophoresis system, the Genomics Core offers services for evaluating the quantity and quality of both DNA and RNA samples in a wide range of concentrations and sample types to its users.
Qubit Fluorometer
The Qubit Fluorometer is a small, easy to use instrument used for quantification of DNA and RNA samples. The fluorometric based assays are an industry standard for all Illumina assays and applications. Results are provided in EXCEL format with ng/ul units.
| Qubit Assay Kit Available | Quantitation Range |
|---|---|
| dsDNA BR Assay Kit | 2 – 1000ng |
| dsDNA HS Assay Kit | 0.1 – 100ng |
| RNA BR Assay Kit | 20 – 1000ng |
| RNA HS Assay Kit | 5 – 100ng |
Agilent TapeStation 4150
The Agilent TapeStation 4150 (pictured above) is a fully automated electrophoresis platform used for sizing, quantifying, and assessing quality of DNA and RNA samples. The high sensitivity DNA ScreenTapes allow for the analysis and sizing of fragmented DNA or Next-Gen DNA libraries, while the Genomic DNA ScreenTapes assess samples up to 60,000bp. The RNA kits allow for the assessment of RNA integrity by assigning an RNA quality score (RIN) to each profile generated, on a scale of 1–10 where 10 is fully intact RNA and 1 is completed degraded RNA. The algorithms apply the 28s/18s ratio to generate a score. Results are provided in PDF format.
| Kits Available | Size Range | Quantitation Range |
|---|---|---|
| High Sensitivity D5000 ScreenTape | 100 – 5,000 bp | 10 – 1,000 pg/ul |
| D1000 ScreenTape | 100 – 5,000 bp | 0.1 – 50 ng/ul |
| D5000 ScreenTape | 35 – 1,000 bp | 0.1 – 50 ng/ul |
| Genomic DNA ScreenTape | 200 to > 60,000 bp | 10 – 100 ng/ul |
| High Sensitivity RNA ScreenTape | RIN 28S / 18S | 500 – 10,000 pg/ul |
| RNA ScreenTape | RIN 28S / 18S | 25 – 500 ng/ul |
E-Gel Agarose Gel Electrophoresis System
The E-Gel Agarose Gel Electrophoresis System is a completely bufferless system for the analysis of DNA length and quality. In the Genomics Core, it is used mainly for the assessment of genomic DNA and PCR products. Results are provided in PNG format.
QC Only Sample Submission
| 1. | 2. | 3. |
|---|---|---|
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The volume required for analysis is dependent upon the services chosen. Qubit ONLY: 3 uL min Full QC - Qubit & FA: 5 uL min More is always appreciated. |
Submit samples in 1.5ml tubes, clearly labeled with sample name, date, and your name/initials. Sample name on tube and iLab request form should match. No strip tubes. |
Do not submit your entire sample. We cannot store leftover sample. If you do not wish for your samples to be discarded please make your request known in BOLD letters and please retrieve the samples within 7 days after results have been e-mailed. |