Pricing

Next-Gen Sequencing Services

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Prices listed include necessary reagents and processing fees for the run only. All samples run on the Illumina instruments must first undergo final library quality control (QC) performed by the Core. Library Prep, QC, and other services requested are not included the cost of the run and will be charged separately. Prices updated August 2019. 

NextSeq 550 v2.5 flowcells

Description: 400 million clusters (High Output flowcell), 130 million clusters (Mid Output kits). 

All pricing listed is for all CWRU affiliates and non-profit affiliates. For "for-profit" pricing, contact Simone Edelheit.

Type of run # of Cycles

Mid Output (130M clusters)

High Output (400M clusters)
Single Read 75 bp $1,400 $1,700
       
Paired-end 75 bp $1,400 $3,200
  150 bp $2,200 $5,200

MiSeq – v2 & v3 flowcells

Description: One lane flowcell with maximum 15M read output (v2 reagents) or 25M read output (v3 reagents).

All pricing listed is for all CWRU affiliates and non-profit affiliates. For "for-profit" pricing, contact Simone Edelheit.

Run Type  MS v3 (25M clusters) MS v2 (15M clusters) MS v2 - Pico (4M clusters) MS v2 - Nano (1M clusters)
1 x 50 bp $1,100 $950 $700 $500
1 x 150 bp $1,100 $1,400 $700 $500
2 x 75 bp $1,100 $1,400 $700 $500
2 x 150 bp $1,995 $1,400 $700 $500
2 x 250 bp $1,995 n/a n/a n/a

DNA & RNA Library Prep Services

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Prices include necessary reagents, processing fees, and QC of starting material and final libraries. The cost of sequencing is not included and will be charged separately. Discounts on larger projects are only applied when samples are submitted on the same day. Prices updated August 2019.

All pricing listed is for all CWRU affiliates and non-profit affiliates. For profit, add 60%. 

DNA Library Description Price / Library
TruSeq DNA PCR Free < 24 Libraries $100
    (1 ug starting material) > 24 Libraries $75
Nextera XT DNA < 24 Libraries $100
    (1 ng starting material) > 24 Libraries $75
Whole Exome Library < 12 Libraries $250
     > 12 Libraries $150
CRISPR-Cas 9 Validation max. 288 amplicons per pool $10
16S Microbiome Analysis V3/V4 region, cost per 96-well plate  $1,500 / 96-well plate
    V3/V4 region, cost per half-plate $1,000 / half-plate

 

RNA Library Description Price/Library
TruSeq Stranded Total RNA – RiboZero Gold Library #1-8 $250
    (1 ug in 20 uL) Library #9-96 $175
Low Input RNA Library Prep contact the Core TBD

Quality Control and Size Selection Services

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All pricing listed is for all CWRU affiliates and non-profit affiliates. For profit, add 60%. Prices updated August 2019.

Service Description Price
QUBIT concentration of total RNA, DNA, and/or prepared library $4.00/sample
DNA QC Agilent DNA HS Bioanalyzer Chip – incl. Qubit $18.00/sample
  eGel – incl. Qubit $6.00/sample
RNA QC Agilent Bioanalyzer Pico / Nano RNA Chip – incl. Qubit $18.00/sample
Final Library QC Agilent DNA HS Bioanalyzer – incl. Qubit (per library) $18.00/sample
  qPCR Analysis – incl. Qubit (per library and/or final library pool) $35.00/sample and/or pool
MiSeq Library QC Run Illumina MiSeq v2 Library QC run $500.00/run
DNA Size Selection / Shearing Covaris S2 $22.00/tube
DNA Size Selection Pippin Prep - price per cartridge (1-5 samples) $75.00/cartridge
Pooling Mix up to 12 libraries with unique tags into one pool $25.00
  Mix up to 24 libraries with unique tags into one pool $50.00
  Mix up to 48 libraries with unique tags into one pool  $75.00
  Mix up to 96 libraries with unique tags into one pool $100.00
Bead Clean-up Final Library Bead Clean-up - incl. Qubit and Bioanalzyer $25.00/sample

Other Services

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For Illumina genotyping/gene expression projects prices include only processing fee. Reagents are extra and should be purchased directly through Illumina. Prices updated July 2017.

All pricing listed is for all CWRU affiliates and non-profit affiliates. For profit, add 48.5%.

Other Services Using Applied Biosystems 3730 DNA Analyzer

Service Details Price
 Sanger Sequencing per reaction $8.00
  per reaction – full 96 well plate $5.00
  EXO/SAP PCR clean-up $1.00
 Microsatellite/SNPs/SNaPshot per reaction $5.00
  per reaction – full 96 well plate $3.00

Other Services Using Illumina HiScan Analyzer

BeadArray Service Array Type (not all-inclusive) Price / Chip
Infinium Genotyping Arrays  HumanOmni5, HumanOmni2.5 Exome, HumanOmni Express, InfiniumHD iSelect… $250
 Infinium Methylation Arrays Infinium Methylations450K (incl. Zymo EZ DNA Methylation prep) $350