Next-Gen Sequencing Services
Submit a Request
Prices listed include necessary reagents and processing fees for the run only. All samples run on the Illumina instruments must first undergo final library quality control (QC) performed by the Core. Library Prep, QC, and other services requested are not included the cost of the run and will be charged separately. Prices updated July 2021.
NextSeq 550 v2.5 flowcells
Description: 400 million clusters (High Output flowcell), 130 million clusters (Mid Output kits).
All pricing listed is for all CWRU affiliates and non-profit affiliates. For "for-profit" pricing, contact Simone Edelheit.
| Type of run | # of Cycles |
Mid Output (130M clusters) |
High Output (400M clusters) |
|---|---|---|---|
| Single Read | 75 bp | $1,500 | $1,900 |
| Paired-end | 75 bp | $1,500 | $3,400 |
| 150 bp | $2,300 | $5,400 |
MiSeq – v2 & v3 flowcells
Description: One lane flowcell with maximum 15M read output (v2 reagents) or 25M read output (v3 reagents).
All pricing listed is for all CWRU affiliates and non-profit affiliates. For "for-profit" pricing, contact Simone Edelheit.
| Run Type | MS v3 (25M clusters) | MS v2 (15M clusters) | MS v2 - Micro (4M clusters) | MS v2 - Nano (1M clusters) |
|---|---|---|---|---|
| 1 x 50 bp | $1,250 | $1050 | $700 | $500 |
| 1 x 150 bp | $1,250 | $1,400 | $700 | $500 |
| 2 x 75 bp | $1,250 | $1,400 | $700 | $500 |
| 2 x 150 bp | $2,200 | $1,400 | $700 | $500 |
| 2 x 250 bp | $2,200 | n/a | n/a | n/a |
DNA & RNA Library Prep Services
Prices include necessary reagents, processing fees, and QC of starting material and final libraries. The cost of sequencing is not included and will be charged separately. Discounts on larger projects are only applied when samples are submitted on the same day. Prices updated July 2021.
All pricing listed is for all CWRU affiliates and non-profit affiliates. For profit, add 60%.
| DNA Library | Description | Price / Library |
|---|---|---|
| KAPA DNA PCR Free | < 24 Libraries | $125 |
| (250 ng starting material) | > 24 Libraries | $100 |
| Nextera XT DNA | < 24 Libraries | $100 |
| (1 ng starting material) | > 24 Libraries | $75 |
| Whole Exome Library | < 12 Libraries | $250 |
| > 12 Libraries | $200 | |
| CRISPR-Cas 9 Validation | max. 288 amplicons per pool | $10 |
| 16S Microbiome Analysis | V3/V4 region, cost per 96-well plate | $1,750 / 96-well plate |
| V3/V4 region, cost per half-plate | $1,250 / half-plate |
| RNA Library | Description | Price/Library |
|---|---|---|
| TruSeq Stranded Total RNA – RiboZero Gold | Library #1-8 | $250 |
| (1 ug in 20 uL) | Library #9-96 | $175 |
| NEBNext Ultra II Directional RNA Prep | Library #1-8 | $200 |
| (down to 20 ng input) | Library #9-96 | $175 |
| 10X Single Cell 3' Gene Expression | contact Mark Cameron | mjc230@case.edu |
Quality Control and Size Selection Services
All pricing listed is for all CWRU affiliates and non-profit affiliates. For profit, add 60%. Prices updated July 2021.
| Service | Description | Price |
|---|---|---|
| QUBIT | concentration of total RNA, DNA, and/or prepared library | $5.00/sample |
| DNA QC | Agilent DNA HS Bioanalyzer Chip – incl. Qubit | $18.00/sample |
| eGel – incl. Qubit | $7.00/sample | |
| RNA QC | Agilent Bioanalyzer Pico / Nano RNA Chip – incl. Qubit | $18.00/sample |
| Final Library QC | Agilent DNA HS Bioanalyzer – incl. Qubit (per library) | $18.00/sample |
| qPCR Analysis – incl. Qubit (per library and/or final library pool) | $40.00/sample and/or pool | |
| MiSeq Library QC Run | Illumina MiSeq v2 Library QC run | $500.00/run |
| DNA Size Selection / Shearing | Covaris S2 | $20.00/tube |
| DNA Size Selection | Pippin Prep - price per cartridge (1-5 samples) | $75.00/cartridge |
| Pooling | Mix up to 12 libraries with unique tags into one pool | $25.00 |
| Mix up to 24 libraries with unique tags into one pool | $50.00 | |
| Mix up to 48 libraries with unique tags into one pool | $75.00 | |
| Mix up to 96 libraries with unique tags into one pool | $100.00 | |
| Bead Clean-up | Final Library Bead Clean-up - incl. Qubit and Bioanalzyer | $25.00/sample |
Other Services
For Illumina genotyping/gene expression projects prices include only processing fee. Reagents are extra and should be purchased directly through Illumina. Prices updated July 2021.
All pricing listed is for all CWRU affiliates and non-profit affiliates. For profit, add 60%.
Other Services Using Applied Biosystems 3730 DNA Analyzer
| Service | Details | Price |
|---|---|---|
| Sanger Sequencing | per reaction | $8.00 |
| per reaction – full 96 well plate | $5.00 | |
| EXO/SAP PCR clean-up | $1.00 | |
| Microsatellite/SNPs/SNaPshot | per reaction | $5.00 |
| per reaction – full 96 well plate | $3.00 |
Other Services Using Illumina HiScan Analyzer
| BeadArray Service | Array Type (not all-inclusive) | Price / Chip |
|---|---|---|
| Infinium Genotyping Array | Human Omni5, HumanOmni2.5 Exome, Global Screening Array, Diversity Array... | $300 |
| Infinium Methylation Arrays | Infinium Methylations450K (incl. Zymo EZ DNA Methylation prep) | $400 |