| Next-Gen Sequencing | Library Preparation | Quality Control |
| Illumina BeadArray | Cell Line Authentication | Sanger Sequencing |
Next-Gen Sequencing Services
MiSeq i100 Plus now available!
The CWRU Genomics Core offers sequencing services on Illumina MiSeq i100 Plus, NextSeq 550, and NovaSeq X Plus instruments. Prices listed include necessary reagents and processing fees for the run only. All samples run on the Illumina instruments must first undergo final library quality control (QC) performed by the Core. Library Prep, QC, and other services requested are not included the cost of the run and will be charged separately. Pricing updated July 2026.
NovaSeq X - Plus
- 5, 10 & 25 billion clusters across full flowcells are available in two different kit options: 300 cycle and 100 cycle
- 1.5 billion clusters flowcells are available in 600, 300, and 100 cycles.
- For better pricing - select Shared Flow cell lane - 1.25B-1.6B clusters
- NEW NovaSeq X 5B flowcells now available as of July 2026
NextSeq 550
- Great for mid-range projects (130-400M reads)
- Cost effective solution to small RNA-seq, ChIP-seq studies
MiSeq i100 Plus
- Faster and cheaper runs, with up to 100M reads generated
- Flow cells with 5, 25, 50 & 100 million clusters available
- Max read length: 2 x 500 bp
- Best for 18S / ITS and amplicon sequencing, small genomes, and low throughput preps
- Used as QC tool before sequencing larger throughput
All pricing listed is for all CWRU affiliates and non-profit affiliates only. For additional pricing, contact Simone Edelheit. Updated 07/2026
| Sequencing Strategy | # of Reads | Run Type | Pricing |
|---|---|---|---|
| NovaSeq X 1.5B - Shared F/C | 800M (1 lane, of 2) | ANY | $1,800 |
| NovaSeq X 1.5B - 100 cycle | 1.5 - 2B | PE 50 / 10X | $2,700 |
| NovaSeq X 1.5B - 300 cycle | 1.5 - 2B | PE 150 | $3,500 |
| NovaSeq X Shared F/C - 1 lane | 1.25 - 1.6B | ANY | $1,400 |
| NovaSeq X 5B - 100 cycle | 5 - 6B | PE 50 / 10X | $4,300 |
| NovaSeq X 5B - 300 cycle | 5 - 6B | PE 150 | $5,600 |
| NovaSeq X 10B - 100 cycle | 10 -12B | PE 50 / 10X | $7,800 |
| NovaSeq X 10B - 300 cycle | 10 -12B | PE 150 | $10,000 |
| NovaSeq X 25B - 100 cycle | 25 - 32B | PE 50 / 10X | $15,900 |
| NovaSeq X 25B - 300 cycle | 25 - 32B | PE 150 | $21,000 |
| NextSeq 550 High Output - 75 cycle | 400M | SR 75 | $2,300 |
| NextSeq 550 High Output - 150 cycle | 400M | PE 75 | $4,300 |
| NextSeq 550 Mid Output - 150 cycle | 130M | SR 75 / PE 75 | $1,800 |
| NextSeq 550 Mid Output - 300 cycle | 130M | PE 150 | $2,700 |
| MiSeq i100 - 5M - 300 cycle | 5M | PE 150 | $625 |
| MiSeq i100 - 5M - 600 cycle | 5M | PE 300 | $925 |
| MiSeq i100 - 25M - 100 cycle | 25M | SR 100 / PE 50 | $775 |
| MiSeq i100 - 25M - 300 cycle | 25M | PE 150 | $1,200 |
| MiSeq i100 - 25M - 600 cycle | 25M | PE 300 | $1,475 |
| MiSeq i100 - 25M - 1000 cycle | 25M | PE 500 | $2,200 |
| MiSeq i100 - 50M - 100 cycle | 50M | SR 100 / PE 50 | $1,075 |
| MiSeq i100 - 50M - 300 cycle | 50M | PE 150 | $1,475 |
| MiSeq i100 - 50M - 600 cycle | 50M | PE 300 | $1,850 |
| MiSeq i100 - 100M - 100 cycle | 100M | SR 100 / PE 50 | $1,375 |
| MiSeq i100 - 100M - 300 cycle | 100M | PE 150 | $1,925 |
Library Prep Services
The CWRU Genomics Core offers a wide range of library prep services for both RNA and DNA. Prices include necessary reagents, processing fees, and QC of starting material and final libraries. The cost of sequencing is not included and will be charged separately. Discounts on larger projects are only applied when samples are submitted on the same day. Pricing updated February 2025.
All pricing listed is for all CWRU affiliates and non-profit affiliates only.
| Library Type (DNA) | Description | Price / Library |
|---|---|---|
| Whole Genome Library - PCR Free | <24 samples | $125 |
| >24 samples | $100 | |
| Whole Genome Library | <24 samples | $100 |
| >24 samples | $75 | |
| Human Exome Library | <12 samples | $250 |
| >12 samples | $200 | |
| 16S / ITS / custom amplicon | Full plate (up to 96 reactions) | $1,750 |
| Half plate (up to 48 reactions) | $1,250 | |
| Amplicon - Index ONLY | Full plate (up to 96 reactions) | $1,250 |
| ChIP-seq / CUT&RUN | Submit targeted DNA | $175 |
| CRISPR-Cas 9 Validation | Pool up to 288 amplicons | $15 |
| Library Type (RNA) | Descriptions | Price / Library |
|---|---|---|
| Total RNA Library | Library #1-8 | $200 |
| Library #9-96 | $175 | |
| mRNA Library | Poly-A enrichment | $150 |
| RIP-seq | Submit enriched RNA | $200 |
| Small RNA-seq | Submit total or enriched RNA | $200 |
| 10X Single Cell Platform | Contact AFGC | mjc230@case.edu |
Quality Control Services
The CWRU Genomics Core accepts samples for DNA and RNA QC at various stages of processing. Samples are analyzed on the Agilent Fragment Analyzer using the appropriate kit or other specified instruments. Prices updated February 2025.
| Service | Description | Price |
|---|---|---|
| QUBIT | Concentration of total RNA, DNA, and/or prepared library | $5.00/sample |
| DNA QC | Agilent TapeStation – incl. Qubit | $16.00/sample |
| eGel – incl. Qubit | $7.00/sample | |
| RNA QC | Agilent TapeStation – incl. Qubit | $16.00/sample |
| Final Library QC | Agilent TapeStation – incl. Qubit | $16.00/sample |
| qPCR Analysis – incl. Qubit | $40.00/reaction | |
| MiSeq Library QC Run | Illumina MiSeq v2 Nano flowcell | $500.00/run |
| DNA Shearing | Covaris S2 | $20.00/tube |
| DNA Size Selection | Pippin Prep - price per cartridge (1-5 samples) | $75.00/cartridge |
| Pooling | Mix up to 12 libraries with unique tags into one pool | $25.00 |
| Mix up to 24 libraries with unique tags into one pool | $50.00 | |
| Mix up to 48 libraries with unique tags into one pool | $75.00 | |
| Mix up to 96 libraries with unique tags into one pool | $100.00 | |
| Bead Clean-up | Final Library Bead Clean-up - incl. Qubit and Bioanalzyer | $25.00/sample |
Illumina Genotyping/Methylation BeadArray Services
For Illumina genotyping/gene expression projects prices include only processing fee. Reagents are extra and should be purchased directly through Illumina. Prices updated February 2025.
| BeadArray Service | Description | Price / Chip |
|---|---|---|
| Infinium Genotyping Array | up to 12 samples per chip | $300 |
| up to 24 samples per chip | $400 | |
| Infinium MethylationEPIC Array | incl. Zymo EZ DNA Methylation Prep | $400 |
| FFPE processing | $200 |
Cell Line Authentication
As of July 1, 2026 Cell Line Authentication services have been discontinued. Please reach out to the core for recommended providers.
As of July 1, 2024 standard Sanger sequencing and genotyping/microsatellite services have been discontinued.
All pricing listed is for all CWRU affiliates and non-profit affiliates only.