Our staff has over 30 years of combined experience with different RNA library prep kits, from high quality samples to FFPE. We provide free consultations to all users before submitting a project to make sure the right experimental design is chosen. The core partners with the Applied Functional Genomics Core (Director: Dr. Mark Cameron) from the Department of Population and Quantitative Health Sciences, to provide RNA-seq analysis from single-cell and ultra-low RNA input amounts. Contact the the AFGC for more details on their services and pricing.
For additional information not found below or on the Genomics Core Pricing page, please contact CWRUgenomics@case.edu.
Included in the cost of an NGS RNA library prep:
- RNA Quality Control - Qubit for concentration (ng/ul) and the Agilent TapeStation RNA tape to determine the RNA Quality Number (RIN)
- Reagent costs
- Library preparation / Labor
- Final Library QC, pooling, and qPCR
Sample Submission
| Library Prep | Recommended Concentration | Required Concentration | Total Volume (uL) |
|---|---|---|---|
| Bulk RNA-seq (total RNA) | 50 ng/uL | 5 ng/uL | 20 |
| Bulk RNA-seq (mRNA) | 50 ng/uL | 5 ng/uL | 20 |
| small RNA-seq | 20 ng/uL | 1 ng/uL | 20 |
| Exosome RNA-seq | 20 ng/uL | 5 uL RNA Serum/Plasma | 20 |
| RIP-seq | 50 ng/uL | 10 ng/uL | 20 |
For a list of available preps and pricing, view our Pricing page.