Evaluating DNA and RNA
Using a combination of a Qubit Fluorometer, the Agilent 2100 Bioanalyzer, Advanced Analytical Fragment Analyzer, and the Invitrogen E-gel Agarose Gel Electrophoresis system, the Genomics Core offers services for evaluating the quantity and quality of both DNA and RNA samples in a wide range of concentrations and sample types to its users.
The Qubit Fluorometer is a small, easy to use instrument used for quantification of DNA and RNA samples. The fluorometric based assays are an industry standard for all Illumina assays and applications. Results are provided in EXCEL format with ng/ul units.
|Qubit Assay Kit Available||Quantitation Range|
|dsDNA BR Assay Kit||2 – 1000ng|
|dsDNA HS Assay Kit||0.1 – 100ng|
|RNA BR Assay Kit||20 – 1000ng|
|RNA HS Assay Kit||5 – 100ng|
Agilent 2100 Bioanalyzer / Advanced Analytical Fragment Analyzer
The Agilent 2100 Bioanalyzer and Advanced Analytical Fragment Analyzer are both micro-fluidics based platforms for sizing, quantification, and quality control of DNA and RNA samples. The high sensitivity DNA kits allows for the analysis of fragmented DNA or DNA libraries. The RNA kits allow for the assessment of RNA integrity by assigning an RNA quality score (RIN/RQN) to each profile generated, on a scale of 1–10 where 10 is fully intact RNA and 1 is completed degraded RNA. The algorithms apply the 28s/18s ratio to generate a score. Results are provided in PDF format.
|Kits Available||Quantitation Range|
|Agilent High Sensitivity DNA Kit / AATI High Sensitivity NGS Fragment Analysis Kit||Low concentrated dsDNA from 50 – 7000bp|
|Agilent RNA 6000 Nano Kit / AATI Standard Sensitivity RNA Analysis kit||Total and mRNA of 25 – 500ng/ul|
|Agilent RNA 6000 Pico Kit||Total and mRNA of 200 – 5000pg/ul|
E-Gel Agarose Gel Electrophoresis System
The E-Gel Agarose Gel Electrophoresis System is a completely bufferless system for the analysis of DNA length and quality. In the Genomics Core, it is used mainly for the assessment of genomic DNA and PCR products. Results are provided in PNG format.
QC Only Sample Submission
The volume required for analysis is dependent upon the services chosen.
Qubit ONLY: 3 ul min
Full QC - Qubit & BioAnalyzer: 5 ul min
More is always appreciated.
Submit samples in 1.5ml tubes, clearly labeled with sample name, date, and your name/initials. Sample name on tube and iLab request form should match. No strip tubes.
Do not submit your entire sample. We cannot store leftover sample.
If you do not wish for your samples to be discarded please make your request known in BOLD letters and please retrieve the samples within 7 days after results have been e-mailed.