Instrumentation

Orbitrap Elite™ Hybrid Ion Trap-Orbitrap Mass Spectrometer 

Orbitrap Elite
ThermoFisher Scientific

 

Analyze demanding proteomics, metabolomics, lipidomics and metabolism samples in greater depth using the Thermo Scientific™ Orbitrap Elite™ Hybrid Ion Trap-Orbitrap Mass Spectrometer. This instrument combines a high-field Thermo Scientific™ Orbitrap ™ mass analyzer and Thermo Scientific™ Velos Pro™ ion trap technology to deliver very high resolution, speed, sensitivity and complementary fragmentation information.

 

 

Performance

  • Very high resolving power—up to 240,000 FWHM—facilitates analysis of low-abundance components in complex samples and resolves isobaric species and isotope fine structures with confidence
  • Ion optics with novel neutral-blocking technology and an advanced detection system increase system robustness and quantification capabilities
  • Advanced signal processing combined with fast scanning ensures UHPLC compatibility
  • Multiple fragmentation techniques—CID, HCD and optionally ETD—provide versatility and complementary data
  • Innovative pre-amplifier electronics improve sensitivity
  • Parallel MS and MSn capability speeds analyses

 

 

6540 UHD Q-TOF System Features

  • Agilent Jet Stream thermal-gradient-focusing technology provides high femtogram MS/MS sensitivity and
    Agilent 6540 Q-TOF
    identification of trace level compounds.
  • Up to 500 ppb mass accuracy improves confidence and reduces false positives.
  • Fast data acquisition rates of up to 20 spectra/second ensure maximum compatibility with Agilent 1290 UHPLC and high-through put methods.
  • Up to five orders of in-spectrum dynamic range improves detection of trace-level targets in the presence of high-abundance compounds.
  • Up to 40,000 mass resolution distinguishes target analytes from intereferences.
  • High-definition isotopic fidelity confirms molecular formulas with greater confidence.

 

 

 

 

 

 

 

 

 

 

 

ThermoFisher Triple Quadrapole Mass Spectrometer

The TSQ Quantum Ultra EMR™ offers higher resolution, higher resolution precursor selection, and an Extended Mass Range of up to 3000 daltons. This extended mass range capability allows high-resolution analysis of a whole new

TSQ Quantum
ThermoFisher Scientific

class of biopolymers including peptides, polysaccharides, intact proteins, and oligo nucleotides. It combines the benefits of the TSQ Quantum Ultra, including constant neutral loss scanning, parent scanning, high-resolution precursor ion selectivity, SRM and H-SRM to deliver a complete solution for the proteomics and large molecule research community.

 

 

 

 

 

 

 

 

High-Confidence Results, Quickly

  • HRAM and full-scan capabilities capture all sample data, all the time, enabling retrospective data analysis without need to re-run samples
  • Resolving power up to 140,000 FWHM eliminates isobaric interferences, increasing confidence in results when analyzing samples in complex matrices
  • Better than 1ppm mass accuracy in full-scan and all-ion fragmentation (AIF) modes ensures confident compound
    Q Exactive Mass Spectrometer
    identification
  • Fast scanning at 12hz and spectral multiplexing suited to UHPLC applications
  • Rapid polarity switching maximizes information obtained
  • AIF and multiple dissociation techniques—in-source CID and HCD—aid in compound identification
  • Extended mass range to 6,000m/z enhances detection of singly charged small molecules and biomolecules
  • More than four orders of magnitude intrascan dynamic range, along with femtogram-level sensitivity, allow detection of trace-level and high-abundance compounds in the same scan