Small molecules and their analogs are chromatographically separated from endogenenous interferences over a reverse phase C18 column. SRM MS data is processed using Pinpoint software (Thermo). The ratio of drug to its analog signal intensity is plotted against the calibration curve to determine drug concentration in clinical samples.
The Core using the following mass spectrometery instrumentation for these assays:
- Waters nanoAcquity UPLC interfaced to a Quantum Ultra mass spectrometer
- Dionex Ultimate 3000 UPLC interfaced to a TSQ Quantiva mass spectrometer
- Agilent 1290 UPLC interfaced to 6540 Q-TOF
- Thermo Fisher Q Exactive Hybrid Quadrapole-Orbitrap mass spectrometer
During Hepatitis C Virus (HCV) Infection and HCV-HIV Coinfection, an Elevated Plasma Level of Autotaxin Is Associated With Lysophosphatidic Acid and Markers of Immune Activation That Normalize During Interferon-Free HCV Therapy.
Kostadinova L, Shive CL, Judge C, Zebrowski E, Compan A, Rife K, Hirsch A, Falck-Ytter Y, SchlatzerDM, Li X, Chance MR, Rodriguez B, Popkin DL, Anthony DD.
J Infect Dis. 2016 Nov 1;214(9):1438-1448. Epub 2016 Aug 17.