Fixed Brain Biopsy | |
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CPT Code | 88307 & 88342 |
Ordering Recommendation |
This test can aid in determining the type of prion disease. Different types of prion disease have different distribution patterns of the abnormal protein. Please note: cortical brain biopsies do not rule out the possibility of Fatal Insomnia (FI). |
Methodology |
Histological Analysis:
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Available STAT | No. |
Analytic Timei | 5 business days. |
Submit With Order |
NPDPSC Test Request Form. Please fill out the insurance information if the center is to bill the patient directly. |
Specimen Requirements | |
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Patient Preparation | Patients under 12 years of age will require documentation of medical necessity and testing will be approved by NPDPSC Director. |
Specimen Type | Brain Tissue (gray matter only). |
Preferred Container | 10% buffered formalin. |
Other Accepted Forms |
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Specimen Preparation |
Place at least 0.5g of gray matter tissue in 10% buffered formalin for at least 24 hours. |
Storage/Transport |
- Unstained Slides: Should be placed in plastic slide holders, then placed in a sealed primary bag and mailed in a FedEx Clinical Pack envelope. - Cassettes/Blocks: Should be placed in a sealed primary bag and mailed in a bubble wrap envelope. - Fixed Tissue: Should be shipped in a pre-filled 10% neutral buffered formalin container. Place the labeled specimen in a leak-proof plastic bag, and place the bag in a Styrofoam carton with absorbent material. Please note: Please call the laboratory at |
Unacceptable Conditions |
White matter, tumor, mass, or dura are not acceptable for prion diagnosis and subtyping. Additionally, specimen quality could affect the ability to rule out prion disease. Avoid freezing of fixed tissue, which may cause artifact. |
Remarks | Complete the required test request forms linked above. |
Stability |
Up to 7 days at room temperature (in formalin). |
Specimen Reporting | |
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Reference Range | Negative. |
Critical Results | None. |
Histopathology Results | Negative or Positive. |
References
i. Expected turnaround-time (business days) for results, beginning when the center receives the specimen.
Frozen Brain Biopsy | |
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CPT Code | 88372 |
Ordering Recommendation |
This test can aid in determining the type of prion disease. Different types of prion disease have different distribution patterns of the abnormal protein. Please note: cortical brain biopsies do not rule out the possibility of Fatal Insomnia (FI). |
Methodology | Western Blot : Digestion of brain tissue containing the pathogenic prion protein yields a 21kD peptide (Type 1) or a 19kD peptide (Type 2), both of which are detected. The resulting peptides are separated on the basis of molecular size by electrophoresis. |
Available STAT | No. |
Analytic Timei | 5 business days. |
Submit With Order |
NPDPSC Test Request Form. Please fill out the insurance information if the center is to bill the patient directly. |
Specimen Requirements | |
---|---|
Patient Preparation | Patients under 12 years of age will require documentation of medical necessity and testing will be approved by NPDPSC Director. |
Specimen Type | Brain Tissue (gray matter only). |
Preferred Container | Sterile specimen cup. |
Storage/Transport |
Please note: Always send frozen specimens separately from fixed specimens to avoid freezing of fixed tissue, which may cause artifact. Please call the laboratory at |
Unacceptable Conditions |
White matter, tumor, mass or dura are not acceptable for prion diagnosis and subtyping. Additionally, specimen quality could affect the ability to rule out prion disease. |
Remarks | Complete the required test request forms linked above. |
Stability |
Up to 7 days at least -20°C. |
Specimen Reporting | |
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Reference Range | Negative. |
Critical Results | None. |
Histopathology Results | Negative or Positive. |
References
i. Expected turnaround-time (business days) for results, beginning when the center receives the specimen.