A successful host-immune response is achieved when a fine-tuned balance of pro-and anti-inflammatory signals is established. Macrophages provide front-line defense against pathogen with enhanced immune responses but also help restore tissue homeostasis after acute inflammation. I am interested in identifying signaling pathways and cytokines that help strike a balance of pro-inflammatory mechanisms vs. anti-inflammatory responses in macrophages upon exposure to M. tuberculosis (Mtb).
- Shukla S, Richardson ET, Drage MG, Boom WH and Harding CV. Mycobacterium tuberculosis lipoprotein and lipoglycan binding to Toll-like receptor 2 correlates with agonist activity and functional outcomes. Infect. Immun. 2018, Sep 21; 86(10). Pil: e00450-18. doi: 10.1128/IAI.00450-18.
- Athman JJ, Sande OJ, Groft S, Nagy NU, Wearsch PA, Richardson ET, Rojas R, Boom WH*, Shukla S* and Harding CV. (* Co-communicating authors). Mycobacterium tuberculosis membrane vesicles inhibit T cell activation. Journal of Immunology, 2017, Mar 1; 18(5): 2028-37
- Nguyen TP, Shukla S, Asaad R, Freeman ML, Lederman MM, Harding CV and Sieg SF. Responsiveness to IL-7 but not to IFN-a is diminished in CD4+ T cells from treated HIV infected patients who experience poor CD4+ T-cell recovery. AIDS, 2016, Aug 24; 30(13), 2033-42.
- Richardson ET, Shukla S, Nagy N, Beck RC, Zhou L, Landreth GE and Harding CV. ERK signaling is essential for macrophage development. PLoS One. 2015, Oct; 10(10): e0140064
- Richardson ET, Shukla S, Sweet DR, Wearsch PA, Tsichlis PN, Boom WH and Harding CV. TLR2-dependent ERK signaling in Mycobacterium tuberculosis-infected macrophages drives anti-inflammatory responses and inhibits Th1 polarization of responding T cells. Infect. Immun. 2015, Jun; 83(6) 2242-54
- Shukla S, Richardson ET, Athman JJ, Shi L, Wearsch PA, McDonald D, Banaei N, Boom WH, Jackson M and Harding CV. Mycobacterium tuberculosis lipoprotein LprG binds lipoarabinomannan and determines its cell envelope localization to control phagolysosomal fusion. PLoS Pathogen, 2014 Oct 30, 10(10); e1004471.
- Drage MG, Tsai HC, Pecora ND, Cheng TY, Arida AR, Shukla S, Rojas RE, Seshadri C, Moody DB, Boom WH, Sacchettini JC, Harding CV. Mycobacterium tuberculosis lipoprotein LprG (Rv1411c) binds triacylated glycolipid agonists of Toll-like receptor 2. Nature Structural & Molecular Biology, 2010, Sep, 17(9) 1088-95.
- Shukla S and Fisher, SA. Tra2β as a novel mediator of vascular smooth muscle diversification. Circulation Research, 2008 Aug 29, 103(5): 485-92.
- Shukla S, Konczak D, Breathnach R and Fisher SA. Competition of PTB with TIA proteins for binding to a U-rich cis-element determines tissue- specific splicing of the myosin phosphatase targeting subunit 1. RNA, 2005 Nov, 11(11) 1725-36.
- Shukla S, Dirksen WP, Joyce KM, Le Guiner-Blanvillain C, Breathnach R and Fisher SA. TIA proteins are necessary but not sufficient for the tissue-specific splicing of the Myosin Phosphatase Targeting Subunit-1. Journal of Biological Chemistry, 2004, 279(14) 13668-76.