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Instrumentation

Cell Sorting

BD Biosciences AriaII - SORP

  • 5 lasers (Blue, Green, Violet, UV, Red) excitation
  • Filter emission (18 colors): B695/40; B515/20; G780/60; G695/40; G670/30; G610/20;
  • G575/26; V780/60; V710/50; V670/30; V610/20; V525/50; V450/50; UV740/35; UV
  • 379/28; R780/60; R730/45; R670/30)
  • Collection standard formats: 
    • Tubes 
      • microfuge tubes (1-4 populations)
    • 1 2x75mm 5mL tubes (1-4 populations)
      • 15 mL tubes (1-2 populations)
    • Multi-well plates (1 population)
      • 6, 12, 24, 48, 96, or 384 wells

BD Biosciences Symphony S6

  • 5 lasers (Blue, Violet, UV, Red, Yellow-Green) excitation
  • Filter emission (30 colors):  BB515/20; BB630/20;BB670/30; BB710/50; BB750/30;
  • BB780/60 V431/28; V470/14; V586/15; V610/20; V670/30; 710/50; V750/30; V780/60;
  • UV450/50; UV515/30; UV586/15; UV610/20; UV670/30; UV 740/35; UV 820/60;  UV
  • 379/28; R780/60; R730/45; R670/30; YG 586/15; YG 610/20; YG 670/30; YG 710/50;
  • 780/60)
  • Collection standard formats: 
    • Tubes 
      • microfuge tubes (1-4 populations)
    • 1 2x75mm 5mL tubes (1-6 populations)
      • 15 mL tubes (1-4 populations)
    • Multi-well plates (1 population)
      • 6, 12, 24, 48, 96, or 384 wells

Analysers

BD Biosciences LSR II

  • 5 lasers (Blue, Yellow-Green, Violet, UV, Red) excitation
  • Filter emission (18 colors): B695/40; B525/20; G780/60; G695/40; G670/30; G610/20; G575/26; V780/60; V710/50; V670/30; V610/20; V525/50; V450/50; UV740/35; UV 379/28; R780/60; R730/45; R670/30)
  • Plate reader

BD Biosciences LSRFortessa

  • 5 lasers (Blue, Green, Violet, UV, Red) excitation
  • Filter emission (18 colors): B695/40; B525/20; G780/60; G695/40; G670/30; G610/20; G575/26; V780/60; V710/50; V670/30; V610/20; V525/50; V450/50; UV740/35; UV 379/28; R780/60; R730/45; R670/30)

Thermo Fisher Attune NxT

  • 4 lasers (Blue, Yellow-Green, Violet, Red) excitation
  • Filter emission (14 colors): B530/30; B950/40; B695/40; YG780/60; YG695/40; YG620/15; YG585/16; V710/50; V603/48; V610/20; V512/25; V440/50; R780/60; R720/30; R670/14)

Imaging Cytometry

Amnis Image Stream flow cytometer

  • 3 lasers (Blue 488 nm, Violet 405 nm,  Orange 785 nm, Red 658 nm) excitation
  • Filter emission (10 colors): 2 Brightfields; B480 - 560 nm; B560 - 595 nm; B660 - 740 nm; B740 - 800 nm and Orange; V420 - 505 nm; V505 - 560 nm; V560 - 595 nm; V595 - 660 nm; V660 - 740 nm and Red; R740 - 800 nm)