Cell Sorting
BD Biosciences AriaII - SORP
FACSAria II Sorp
Five lasers: [UV (355nm, Violet (405nm), Blue (488nm), Green (532nm), Red (642nm)]
18 Emission Optical Filters: Ultra Violet: UV379/28, UV740/35; Violet: V450/50, V525/50, V610/20, V670/30, V710/50, V780/60; Blue: B515/20, B695/40; Green: G575/26, G610/20, G670/30, G695/40, G780/60; Red: R670/30, R730/45, R780/60.
Capable of sorting into cell culture and PCR plates; PCR stipes; 15 ml conical tubes, up to 2 simultaneous collections; 12 X 75 tubes or 1.5 microfuge tubes, up to 4 sample simultaneous collections.
Symphony S6
Five lasers: [UV (355nm, Violet (405nm), Blue (488nm), YG (561nm), Red (642nm)]
30 emisson optical filters: V379/28, UV450/50, UV515/30, UV586/15, UV610/20, UV670/30, UV740/35, UV820/60; V431/28, V470/14, V586/15, V610/20, V670/30, V710/50, V750/30, V780/60; B515/20, B610/20, B670/30, B710/50, B750/30, B780/60;YG586/15, YG610/20, YG670/30, YG710/50, YG780/60;R670/30, R710/50, R780/60.
Equipment able to do small particles. Capable of sorting into cell culture and PCR plates; PCR stipes; 15 ml conical tubes, up to 2 simultaneous collections; 12 X 75 tubes or 1.5 microfuge tubes, up to 6 sample simultaneous collections.
Analysers
BD Biosciences LSRFortessa
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Five lasers: [UV (355nm, Violet (405nm), Blue (488nm), Green (532nm), Red (642nm)]
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18 Emission Optical Filters: Ultra Violet: UV379/28, UV740/35; Violet: V450/50, V525/50, V610/20, V670/30, V710/50, V780/60; Blue: B515/20, B695/40; Green: G575/26, G610/20, G670/30, G695/40, G780/60; Red: R670/30, R730/45, R780/60
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High-Throughput System (HTS) is available for running 96-well plates.
Discover A8 Spectral with Imaging
Five lasers: [UV (355nm, Violet (405nm), Blue (488nm), YG (561nm), Red (642nm)]
The BD FACSDiscover™ A8 Cell Analyzer seamlessly integrates cutting-edge spectral flow cytometry with real-time imaging. By combining innovations such as BD SpectralFX™ Technology and BD CellView™ Image Technology into a single instrument, it offers unparalleled experimental power and simplified workflows to deliver reproducible results and single-cell insights that were previously undetectable. This versatile, robust and reliable instrument comes in a 5-laser configuration (B-R-V-YG-UV) with up to 78 fluorescence detectors and 8 scatter and imaging detectors.
Imaging - Blue Laser: SSC (Imaging) 488/15; Light Loss (Imaging) 488/15; ImgB1 (535) 534/46; ImgB2 (600) 600/60; ImgB3 (790) 788/225. Ultraviolet UV1 (375) 375/20; UV2 (390) 392/14; UV3 (420) 420/20;UV4(440)440/20;UV5 (460)459/18;UV6 (475) 476/16; UV7 (500) 501/16; UV8 (515) 517/16; UV9 (530) 532/16; UV10 (545) 547/16; UV11 (575) 574/16; UV12 (590) 590/16; UV13 (605) 607/18; UV14 (625) 625/18; UV15 (655) 655/18; UV16 (675) 675/18; UV17 (700) 698/26; UV18 (725) 724/26; UV19 (750)752/30; UV20 (780) 782/30; UV21 (810) 812/30; UV22 (845) 844/34.Violet Laser: SSC (Violet) 405/15; Light Loss (Violet) V1 (420) 420/20; V2 (440) 440/20; V3 (460) 459/18; V4 (475) 476/16; V5 (500) 501/16; V6 (515) 517/16; V7 (530) 532/16; V8 (545) 547/16; V9 (575) 574/16; V10 (590) 590/16; V11 (605) 607/18; V12 (625) 625/18; V13 (655) 655/20; V14 (675) 675/20; V15 (700) 698/26; V16 (725) 724/26; V17 (750) 752/30; V18 (780) 782/30; V19 (810) 812/30; V20 (845) 844/34.Blue Laser B1 (500) 501/16; B2 (515) 517/16; B3 (530) 532/16; B4 (545) 547/16; B5 (575) 574/16; B6 (590) 590/16; B7 (605) 607/18; B8 (625) 625/18; B9 (655) 655/20; B10 (675) 675/20; B11 (700) 698/26; B12 (725) 724/26; B13 (750) 752/30; B14 (780) 782/30; B15 (810) 812/30; B16 (845) 844/34. Yellow Green Laser YG1 (575) 574/16; YG2 (590) 590/16; YG3 (605) 607/18; YG4 (625) 625/18; YG5 (655) 655/20; YG6 (675) 675/20; YG7 (700) 698/26; YG8 (725) 724/26; YG9 (750) 752/30; YG10 (780) 782/30; YG11 (810) 812/30; YG12 (845) 844/34. Red Laser R1 (655) 655/20; R2 (675) 675/20; R3 (700) 698/26; R4 (725) 724/26; R5 (750) 752/30; R6 (780) 782/30; R7 (810) 812/30; R8 (845) 844/34.
Microscope: Keyence BZ-X810
Installed Objectives: 2X PlanApo_λ 0.10/8.50mm; 4X PlanApo_λ 0.20/20.00mm; 10X PlanFluor_DL 0.30/15.20mm Ph1; 20X PlanApo_λ 0.75/1.00mm; 40X S_PlanFluor_ELWD 0.60/3.60 - 2.90mm Ph2; 20X S_PlanFluor_ELWD_ADM 0.45/8.20 - 6.90mm Ph1
Available Objectives held in reserve: 40X PlanApo_λ 0.95/0.25 - 0.16mm; 60X PlanApo_λ 1.40/0.13mm Oil
Installed Filter Cubes: Excitation: DAPI 360/40; GFP 470/40; TxRed 560/40 TRITC 545/25. Emission: DAPI 460/50; GFP 525/50; TxRed 630/75 TRITC 605/70
Data Analysis Software
FlowJo – License FlowJo Portal
More information contact: Dr. Karina Carvalho kxi68@case.edu