Instrumentation

Cell Sorting

BD Biosciences Aria

  • 3 lasers; 9 color (blue, green, orange, red, far-red) emission
  • Compatible dyes: Hoechst 333 42, DAPI, Pacific Blue, Pacific Orange, 
    Fluorescein/A488, GFP, PI, AO, 7AAD, PE, A555, Cy3, YFP, PE-Cy5, PE-Cy5.5, 
    PE-Cy7, PerCp, PerCp-Cy7, A647, A700, AP, AP-Cy7
  • Four way sorting; multi-well plate sorting. Sort speed 25,000/s

ICyte Reflection

  • 5 lasers (choice of 2); 4 color (blue, green, orange, red, far-red) emission
  • Compatible dyes: Hoechst 33342, DAPI, Pacific Blue, Pacific Orange, Fluorescein/A488, 
    GFP, PI, AO, 7AAD, PE, A555, Cy3, YFP, PE-Cy5, PE-Cy5.5, PE-Cy7, PerCp, PerCp-Cy7, 
    Texas Red, RFP, A647, A700, AP, AP-Cy7
  • Four way sorting. Flexible, high speed. Sort speed 50,000/s

Flow Cytometry

Millipore Guava

  • 1 blue laser; 2 color (green and red) emission
  • Compatible dyes: Fluorescein/A488, PI, 7AAD
  • This cytometer is mainly used for live/dead cell counting and data are output as events per ml. 
    Does not have light scatter parameters.

BD Biosciences LSR 2

  • 5 lasers; 14 color (blue, green, orange, red, far red) emission
  • Compatible dyes: Hoechst 33342, DAPI, A350, Pacific Blue, Pacific Orange, Fluorescein/A488, GFP, 
    PI, AO, 7AAD, PE, A555, Cy3, YFP, PE-Cy5, PE-Cy5.5, PE-Cy7, PerCp, PerCp-Cy7, A647, A700, AP, AP-Cy7

Imaging Cytometry

Compucyte iCyte laser scanning cytometer (LSC)

  • 3 lasers; 8 color (blue, green, orange, red) emission
  • Compatible dyes: Hoechst 33342, DAPI, Pacific Blue, Pacific Orange, Fluorescein/A488, GFP, 
    PI, AO, 7AAD, PE, A555, Cy3, YFP, PE-Cy5, PerCp, A647, AP
  • This platform accepts chamber slides, standard culture dishes with coverslipped 
    windows (Labtek); optical grade multiwall plates

Amnis Image Stream flow cytometer

  • 3 lasers; X color (blue, green, orange, red) emission
  • Compatible dyes: Hoechst 33342, DAPI, Pacific Blue, Pacific Orange, Fluorescein/A488, 
    GFP, PI, AO, 7AAD, PE, A555, Cy3, YFP, PE-Cy5, PE-Cy5.5, PE-Cy7, PerCp, PerCp-Cy7, A647, AP