Effective March 19, 2020

In order to comply with the best practices guidelines recently issued by the University, The Proteomics and Small Molecule Mass Spectrometry Shared Resource (SR) will be instituting the following approach:

  1. Effective March 19th - We will be conducting a 1-week hold on any new samples that will be analyzed by the SR in order to ensure we complete the current samples in the queue such that if needed we can shut down easily without sacrificing samples.  After this 1-week hold we will be assessing projects on a case by case basis.
  2. Any new projects that need to be conducted in the next 3-4 weeks must be submitted to the Assistant Director, Danie Schlatzer.  The request needs to include an explanation of the critical nature of the experiment and the specific time commitment needed by Core staff.  The request will then be submitted to the SR Director, Mark Chance. Any scheduling of new projects will be determined and communicated by Danie Schlatzer.

The Assistant Core Director and Lab Manager are available by email (dms73@case.edu, kcl4@case.edu) to answer questions.

Proteomics

Services

The overall goal of the Proteomics Shared Resource (SR) is to make advanced mass spectrometry instruments, expertise, and methods for proteome analysis available to all investigators.

Services include the following:

  • Protein Identification
  • Characterization of post-translational modifications
  • Quantitation of protein expression
    • Quantitative Top-Down Proteomics using 2-D gels
    • Quantitative Bottom-Up Proteomics using LC-MS
    • Experimental Design for Quantitative Proteomics
    • Data analysis
  • Bioinformatics analysis
  • Training
  • Independent use
  • Interactome analysis
  • Structural analysis (protein footprinting)

Resources

Case Western Reserve University facility

  • Two Thermo Scientific Orbitrap Elite with a Waters nanoACQUITY UPLC system
  • Thermo Scientific Fourier Transform LTQ mass spectrometer with high resolution and ppm mass accuracy for top-down and bottom-up proteomics capabilities operated with a Dionex UltiMate 3000 Rapid Separation LC (RSLC)
  • Thermo Scientific LTQ Orbitrap XL ETD with a Waters nanoACQUITY UPLC system
  • Thermo Scientific LTQ Orbitrap Velos with a Waters nanoACQUITY UPLC system
  • Thermo Scientific TSQ Quantum Ultra Triple Quad Mass Spectrometer with a Dionex UltiMate 3000 Rapid Separation LC (RSLC)
  • prOTOF 2000 MALDI

Lerner Research Institute facility

  • ThermoFinnigan LTQ

  • ThermoFinnigan LTQ-Orbitrap Elite

More Information

Case Western Reserve University
Center for Proteomics and Bioinformatics
proteomics.case.edu/

Cleveland Clinic Lerner Research Institute
Proteomics Laboratory
https://www.lerner.ccf.org/services/proteomics/

Acknowledgment

Remember to acknowledge the valuable services provided by Cancer Center Shared Resources in your research papers and publications: 
"This research was supported by the Proteomics Shared Resource of the Case Comprehensive Cancer Center (P30CA043703)."

Contact for More Information

CWRU Facility LRI Facility
Location: Case BRB 9th floor Location: LRI Room NN1-25
Director: Mark R. Chance, PhD
216.368.4406
mark.chance@case.edu
Co-Director: Belinda B. Willard, PhD
216.444.7170
willarb@ccf.org 
General Contact: Janna Kiselar, PhD
Assistant Director
216.368.1490
proteomics@case.edu