Services
The Hematopoietic Biorepository and Cellular Therapy Shared Resource (SR) serves as a resource for procurement, processing, storage, banking, analysis, and distribution of cells derived from clinical-grade production, human blood, bone marrow, and umbilical cords.
The SR is comprised of two components:
-
A human hematopoietic biorepository and support services to facilitate basic hematopoietic research
- A clinical Cellular Therapy Lab that manufactures clinical-grade cell products for investigational and standard of care therapies
Facility Objectives
- To procure, de-identify, process and distribute human blood, umbilical cord blood, and bone marrow cells from healthy volunteers and patients to investigators
- To maintain a biorepository of collected samples
- To perform and assist investigators with standard assays to evaluate hematopoiesis and hematopoietic and mesenchymal stem cells
- To support cell therapy-based clinical trials from the proof of concept stage throughout the clinical trial
List of Services
- Procurement and distribution of human hematopoietic cells:
- The SR maintains an active IRB protocol to enable the recruitment of donors and procurement and distribution of de-identified blood and bone marrow samples to research investigators. We also maintain a Biorepository to supply investigators with cryopreserved samples. Sample types obtained include:
- Normal donor blood
- Normal donor bone marrow aspirate and/or biopsy samples
- Umbilical cord blood
- Peripheral mobilized hematopoietic progenitor cells
- Bone marrow mesenchymal stem cells
- Blood and/or bone marrow from patients with a wide variety of hematologic diseases including AML, ALL, CML, CLL, multiple myeloma, and anemia
- The SR maintains an active IRB protocol to enable the recruitment of donors and procurement and distribution of de-identified blood and bone marrow samples to research investigators. We also maintain a Biorepository to supply investigators with cryopreserved samples. Sample types obtained include:
- Processing and analysis of hematopoietic cells:
- The SR assists investigators with the processing and analysis of hematopoietic cells using a variety of methodologies. Examples of some of these procedures include:
- Separation of mononuclear cells by density gradient (Ficoll-Hypaque or Percoll)
- Cell enrichment using CliniMACS magnetic bead separation (ex. CD34+ cell isolation)
- Isolation and expansion of mesenchymal stem cells
- Maintenance of specialized equipment available for research use including a hematology analyzer to perform CBCs
- Performance of colony forming unit assays (CFU) on blood and bone marrow products
- Separation of plasma/serum
- The SR assists investigators with the processing and analysis of hematopoietic cells using a variety of methodologies. Examples of some of these procedures include:
- Production of clinical grade cellular therapy products
- Isolation of specific cell subsets (CD34, CD3, CD14, etc.)
- Expansion of various cell types upon request
- Ex-vivo lentiviral gene transfer
- Support capabilities to transition preclinical studies into clinical trials
- Assistance in the design and preparation of cells for Good Laboratory Practices (GLP) safety studies
- Investigational New Drug Application (IND) preparation
- Sourcing of clinical grade supplies and reagents
- Scale up from research to clinical operations, including preparation of investigational clinical therapy grade cellular products
- GMP training to lab investigators
Resources
Hemavet Hematology Analyzer
Guava Cytometer
Biorad QPCR machine
ISO 7 cleanroom suites (6) each with incubators, centrifuge, biosafety cabinet, microscope, refrigerator
GMP-trained lab personnel
COBE closed system cell washer
Rate controlled freezing chamber
Clinical grade Cytokine stocks: Interleukins 3 and 6, stem cell factor and Flt-3 ligand, thrombopoietin
VarioMACS columns/antibody cocktails for routine separation of CD34+ cells and other cell subsets
Shandon Cytospin
Acknowledgment
Remember to acknowledge the valuable services provided by Cancer Center Shared Resources in your research papers and publications:
"This research was supported by the Hematopoietic Biorepository and Cellular Therapy Shared Resource of the Case Comprehensive Cancer Center (P30CA043703)."
Contact for Information
Location | Wolstein Research Building 6-303 |
Core Contact | Basabi Maitra 216.368.1495 basabi.maitra@case.edu |
Scientific Director | Lan Zhou, MD, PhD Assistant Professor, Pathology lan.zhou@case.edu |
Scientific Co-Director | David N. Wald, MD, PhD 216.368.5668 dnw@case.edu |
Operations Director | Jane Reese Koç, MBA, MT 216.368.1007 jsr5@case.edu |