The Applied Functional Genomics Core (AFGC) partners with the CWRU Genomics Core to offer functional genomic study design and a highly standardized, top-to-bottom platform for conducting RNA isolation, Illumina-based RNA sequencing, and advanced bioinformaticson a wide variety of clinical sample types. We provide workflows that are optimized for many difficult sample types, such as ultra-low input (biopsies, small cell numbers), single cell sequencing (10X Genomics Chromium platform), and our analysis tools can integrate multiple data types, whether clinical or functional, in the bioinformatics analysis to uncover biomarkers or correlates. Our advanced bioinformatics methods include pathway and network analysis, meta-analysis, and “Mixomics” for researchers who require a look at biology across very different studies, especially in correlation with immunophenotyping data.
The Applied Functional Genomics Core also serves as the assay and analytics platform for the Cleveland CFAR Experimental and Analytic Systems Biology Core (EASB). The EASB Core houses the Inter-CFAR Bioinformatic Network to support a unique partnership between the Cleveland CFAR and the University of Miami CFAR, thus providing access to other omic technologies and meta-analysis for external CFAR members, and data integration.