Synchrotron X-ray footprinting provides a method of observing the structure and dynamics of biological macromolecules and their interactions in the solution state (in some cases within living cells) at the single nucleotide or single amino acid level. This method is unique in providing "local" structural information in solution for gaining insight into dynamic processes involving large RNA-protein and protein-protein assemblies at a higher resolution on biologically relevant timescales and under physiological conditions. Synchrotron-based footprinting studies have provided novel insights and approaches for steady state and time-resolved studies of macromolecular dynamics, examining the structure of large macromolecular assemblies, in vivo studies of macromolecules, and even observing functional water dynamics within the context of these biological structures. The XFP beamline at NSLS-II, developed and operated by the CSB in partnership with NSLS-II, is the premier facility in the world dedicated to synchrotron footprinting studies.
National Synchrotron Light Source II, Building 740, 17 BM XFP
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