A new Nikon AXR Laser Scanning Confocal Microscope purchased by the CWRU Center for Excellence on the Impact of Substance Use on HIV, led by Alan Levine, PhD, is available for use by trained faculty. In addition to confocal capabilities, the microscope is able to perform NSPARC super-resolution, near-IR microscopy, and brightfield microscopy. Additionally, there is a separate analysis computer with deconvolution packages.
Current setup and capabilities:
- Point scanning and resonant galvano
- 4x,10x, 20x air objectives
- 60x,100x oil immersion
- 405 nm, 488 nm, 561 nm, 640 nm, 730 nm laser lines and Brightfield
- NSPARC Superresolution detector (dye agnostic diagnostics)
- Separate analysis computer with additional deconvolution package, each computer has some AI machine learning-based tools (remove shot noise, etc).
- Adaptors can handle slides, plates and smaller petri dishes.
Additional modules may be purchased in the future to expand the microscope’s capabilities.
Current rates are $45/hour for use of the microscope and $15/hour for use of the analysis computer. Image collection by the center will be charged at $65/hour, $85/hour for analysis. When presenting your work publicly, please acknowledge 1 P30 DA054557 and the Center for Excellence on the Impact of Substance Use on HIV.
Reservations for the microscope will be followed using the iLab system. Use will be limited to those fully trained by center personnel. To request training, please email ceisu-hiv@case.edu.